G protein-coupled receptor mediated trimethylamine sensing
2009 (English)In: BIOSENSORS and BIOELECTRONICS, ISSN 0956-5663, Vol. 25, no 4, p. 715-720Article in journal (Refereed) Published
Abstract [en]
A new approach for the detection of trimethylamine (TMA) using a recombinant cell line of Xenopus laevis melanophores was developed. The cells were genetically modified to express the mouse trace amine-associated receptor 5 (mTAAR5), a G protein-coupled receptor from the mouse olfactory epithelium, which conferred high sensitivity to TMA. Cellular responses to TMA were analyzed by two different techniques, either by absorbance measurements using a microplate reader or by cellular imaging via an inverted microscope. A focused chemical screen allowed the discovery of additional, previously unknown stimuli of mTAAR5. The developed cell-based sensor demonstrated no sensitivity to trimethylamine N-oxide (TMAO), making it suitable for a straightforward evaluation of TMA levels in fish tissue extracts. For the detection of TMA vapor, the cells were covered with agarose, which allowed for intact cell viability for at least 6 h in air. The developed gas measurement platform was able to detect TMA from I to 100 ppm within 35 min.
Place, publisher, year, edition, pages
2009. Vol. 25, no 4, p. 715-720
Keywords [en]
Cell-based sensor, Trace amine-associated receptor, TAAR5, Trimethylamine detection, Xenopus laevis melanophores
National Category
Engineering and Technology
Identifiers
URN: urn:nbn:se:liu:diva-52835DOI: 10.1016/j.bios.2009.08.012OAI: oai:DiVA.org:liu-52835DiVA, id: diva2:285595
2010-01-122010-01-122015-06-01