liu.seSök publikationer i DiVA
Ändra sökning
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Dark states in oligothiophenes: evidence from fluorescence correlation spectroscopy and dynamic light scattering
Linköpings universitet, Institutionen för fysik, kemi och biologi, Biomolekylär och Organisk Elektronik. Linköpings universitet, Tekniska högskolan.
Experimental Biomolecular Physics, Department of Applied Physics, Royal Institute of Technology, SE-106 91 Stockholm, Sweden.
Linköpings universitet, Institutionen för fysik, kemi och biologi, Organisk Kemi. Linköpings universitet, Tekniska högskolan.ORCID-id: 0000-0002-5582-140X
Experimental Biomolecular Physics, Department of Applied Physics, Royal Institute of Technology, SE-106 91 Stockholm, Sweden.
Visa övriga samt affilieringar
(Engelska)Manuskript (preprint) (Övrigt vetenskapligt)
Abstract [en]

We report studies of the conjugated pentameric oligothiophene derivative p-FTAA, which changes optical properties in aqueous buffers of varying pH and concentration. Using dynamic light scattering, luminescence spectroscopy and fluorescence correlation spectroscopy, we find evidence for the formation of large clusters of p-FTAA in aqueous environment, formation of very large non-emissive clusters, and the presence of at least two dark transient states, one presumably being a triplet state. The clustering of p-FTAA is therefore an important mechanism. This work provides an interpretation of fluorescence spectra used for the detection of misfolding proteins through interaction with p-FTAA.

Nationell ämneskategori
Naturvetenskap
Identifikatorer
URN: urn:nbn:se:liu:diva-54897OAI: oai:DiVA.org:liu-54897DiVA, id: diva2:311084
Tillgänglig från: 2010-04-20 Skapad: 2010-04-20 Senast uppdaterad: 2014-04-08
Ingår i avhandling
1. Conjugated Polyelectrolytes in Interactions with Biomolecules for Supramolecular assembly and Sensing
Öppna denna publikation i ny flik eller fönster >>Conjugated Polyelectrolytes in Interactions with Biomolecules for Supramolecular assembly and Sensing
2010 (Engelska)Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
Abstract [en]

Conjugated polyelectrolytes (CP) show interesting electrical and optical properties for organic electronics as well as for life science applications. Their possibilities of supramolecular assembly with nanowire like misfolded proteins, amyloids, as well as synthetic polypeptides or DNA forming conducting or luminescent nano composites is highly interesting as being a truly bottom up approach for fabrication of OLEDs, photovoltaic’s as well as logic devices. The conformation and aggregation dependent luminescence properties from the special class of CPs, Luminescent conjugated polyelectrolytes (LCP), have been utilised and developed as sensors to follow and study biomolecular interactions, DNA hybridisation, protein-protein interactions and staining of living cell cultures and tissue slides. In this thesis we are bringing the evolution a few steps further by applying new types of experimental techniques, such as light scattering and fluorescence correlation spectroscopy, combined with standard techniques as soft lithography and different spectroscopy techniques, to gain better knowledge of the optical behaviour of LCPs and their interactions with biomolecules. We explore the optical properties and vibronic transitions of LCPs; their ability of resonance energy transfer with LCPs indicating super lightning behaviour; the opposite fluorescence shift when interacting with α-helical rich polypeptides compared to earlier reports of interactions upon staining of β-rich amyloids; and the possibility of LCPs to influence protein aggregation as well as the possibility of fabricating biochips based on LCPs and soft lithography. Here we also show fundamental limitations to patterning using macromolecular fluids, of general relevance to soft lithography and nanoimprint lithography with low viscosity polymers.

Ort, förlag, år, upplaga, sidor
Linköping: Linköping University Electronic Press, 2010. s. 60
Serie
Linköping Studies in Science and Technology. Dissertations, ISSN 0345-7524 ; 1308
Nationell ämneskategori
Naturvetenskap
Identifikatorer
urn:nbn:se:liu:diva-54902 (URN)978-91-7393-408-4 (ISBN)
Disputation
2010-05-14, Planck, Fysikhuset, Campus Valla, Linköpings universitet, Linköping, 14:00 (Engelska)
Opponent
Handledare
Tillgänglig från: 2010-04-20 Skapad: 2010-04-20 Senast uppdaterad: 2014-04-08Bibliografiskt granskad

Open Access i DiVA

Fulltext saknas i DiVA

Personposter BETA

Wigenius, Jens A.Nilsson, PeterInganäs, Olle

Sök vidare i DiVA

Av författaren/redaktören
Wigenius, Jens A.Nilsson, PeterInganäs, Olle
Av organisationen
Biomolekylär och Organisk ElektronikTekniska högskolanOrganisk Kemi
Naturvetenskap

Sök vidare utanför DiVA

GoogleGoogle Scholar

urn-nbn

Altmetricpoäng

urn-nbn
Totalt: 98 träffar
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf