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Monitoring of troponin release from cardiomyocytes during exposure to toxic substances using surface plasmon resonance biosensing
Linköpings universitet, Institutionen för fysik, kemi och biologi, Teknisk biologi. Linköpings universitet, Tekniska fakulteten.
Linköpings universitet, Institutionen för klinisk och experimentell medicin, Klinisk kemi. Linköpings universitet, Hälsouniversitetet. Östergötlands Läns Landsting, Laboratoriemedicinskt centrum, Klinisk kemi.
Linköpings universitet, Institutionen för fysik, kemi och biologi, Teknisk biologi. Linköpings universitet, Tekniska högskolan.ORCID-id: 0000-0001-9711-794X
2010 (engelsk)Inngår i: ANALYTICAL AND BIOANALYTICAL CHEMISTRY, ISSN 1618-2642, Vol. 398, nr 3, s. 1395-1402Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Troponin T (TnT) is a useful biomarker for studying drug-induced toxicity effects on cardiac cells. We describe how a surface plasmon resonance (SPR) biosensor was applied to monitor the release of TnT from active HL-1 cardiomyocytes in vitro when exposed to cardiotoxic substances. Two monoclonal human TnT antibodies were compared in the SPR immunosensor to analyse the TnT release. The detection limit of TnT was determined to be 30 ng/ml in a direct assay set-up and to be 10 ng/ml in a sandwich assay format. Exposure of the cardiomyocytes to doxorubicin, troglitazone, quinidine and cobalt chloride for periods of 6 and 24 h gave significant SPR responses, whereas substances with low toxicity showed insignificant effects (ascorbic acid, methotrexate). The SPR results were verified with a validated immunochemiluminescence method which showed a correlation of r(2)=0.790.

sted, utgiver, år, opplag, sider
Springer Science Business Media , 2010. Vol. 398, nr 3, s. 1395-1402
Emneord [en]
Surface plasmon resonance, Troponin T, Cardiotoxicity, In vitro toxicity, HL-1 cardiomyocytes, Cytotoxicity screening
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Identifikatorer
URN: urn:nbn:se:liu:diva-59731DOI: 10.1007/s00216-010-4041-9ISI: 000281670400026OAI: oai:DiVA.org:liu-59731DiVA, id: diva2:353130
Merknad

The original publication is available at www.springerlink.com: Henrik Andersson, Bertil Kågedal and Carl-Fredrik Mandenius, Monitoring of troponin release from cardiomyocytes during exposure to toxic substances using surface plasmon resonance biosensing, 2010, ANALYTICAL AND BIOANALYTICAL CHEMISTRY, (398), 3, 1395-1402. http://dx.doi.org/10.1007/s00216-010-4041-9 Copyright: Springer Science Business Media http://www.springerlink.com/

Tilgjengelig fra: 2010-09-24 Laget: 2010-09-24 Sist oppdatert: 2019-01-22

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Andersson, HenrikKågedal, BertilMandenius, Carl-Fredrik

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