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A Collagen-Chitosan Hydrogel for Endothelial Differentiation and Angiogenesis
Univ Ottawa, Inst Heart, Div Cardiac Surg, Ottawa, ON K1Y 4W7 Canada.
Univ Ottawa, Inst Heart, Div Cardiac Surg, Ottawa, ON K1Y 4W7 Canada.
Univ Ottawa, Inst Heart, Div Cardiac Surg, Ottawa, ON K1Y 4W7 Canada.
Univ Ottawa, Inst Heart, Div Cardiac Surg, Ottawa, ON K1Y 4W7 Canada.
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2010 (Engelska)Ingår i: TISSUE ENGINEERING PART A, ISSN 1937-3341, Vol. 16, nr 10, s. 3099-3109Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

 Cell therapy for the treatment of cardiovascular disease has been hindered by low cell engraftment, poor survival, and inadequate phenotype and function. In this study, we added chitosan to a previously developed injectable collagen matrix, with the aim of improving its properties for cell therapy and neovascularization. Different ratios of collagen and chitosan were mixed and chemically crosslinked to produce hydrogels. Swell and degradation assays showed that chitosan improved the stability of the collagen hydrogel. In culture, endothelial cells formed significantly more vascular-like structures on collagen-chitosan than collagen-only matrix. While the differentiation of circulating progenitor cells to CD31(+) cells was equal on all matrices, vascular endothelial-cadherin expression was increased on the collagen-chitosan matrix, suggesting greater maturation of the endothelial cells. In addition, the collagen-chitosan matrix supported a significantly greater number of CD133(+) progenitor cells than the collagen-only matrix. In vivo, subcutaneously implanted collagen-chitosan matrices stimulated greater vascular growth and recruited more von Willebrand factor (vWF(+)) and CXCR4(+) endothelial/angiogenic cells than the collagen-only matrix. These results indicate that the addition of chitosan can improve the physical properties of collagen matrices, and enhance their ability to support endothelial cells and angiogenesis for use in cardiovascular tissue engineering applications.

Ort, förlag, år, upplaga, sidor
Mary Ann Liebert , 2010. Vol. 16, nr 10, s. 3099-3109
Nyckelord [en]
CIRCULATING PROGENITOR CELLS; EMBRYONIC STEM-CELLS; GROWTH-FACTOR; IN-VIVO; MYOCARDIAL-INFARCTION; CARDIAC REPAIR; RAT HEARTS; TRACKING; DELIVERY; BINDING
Nationell ämneskategori
Medicin och hälsovetenskap
Identifikatorer
URN: urn:nbn:se:liu:diva-65511DOI: 10.1089/ten.tea.2009.0504OAI: oai:DiVA.org:liu-65511DiVA, id: diva2:396169
Tillgänglig från: 2011-02-09 Skapad: 2011-02-09 Senast uppdaterad: 2013-12-17

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