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Biosynthetic corneal implants for replacement of pathologic corneal tissue: performance in a controlled rabbit alkali burn model
Linköpings universitet, Hälsouniversitetet. Linköpings universitet, Institutionen för klinisk och experimentell medicin, Oftalmiatrik.
Linköpings universitet, Institutionen för klinisk och experimentell medicin, Oftalmiatrik. Linköpings universitet, Hälsouniversitetet. Östergötlands Läns Landsting, Rekonstruktionscentrum, Ögonkliniken US/LiM.ORCID-id: 0000-0003-1079-4361
University of Ottawa Eye Institute.
Emory University School of Medicine.
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2011 (Engelska)Ingår i: Investigative Ophthalmology and Visual Science, ISSN 0146-0404, E-ISSN 1552-5783, Vol. 52, nr 2, s. 651-657Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Purpose: To evaluate the performance of structurally reinforced, stabilized recombinant human collagen-phosphorylcholine (RHCIII-MPC) hydrogels as corneal substitutes in a rabbit model of severe corneal damage.

Methods: One eye each of 12 rabbits received a deep corneal alkali wound. Four corneas were implanted with RHCIII-MPC hydrogels. The other eight control corneas were implanted with either allografts or a simple crosslinked RHCIII hydrogel. In all cases, 6.25 mm diameter, 350 µm thick buttons were implanted by anterior lamellar keratoplasty to replace damaged corneal tissue. Implants were followed for nine months by clinical examination and in vivo confocal microscopy, after which implanted corneas were removed and processed for histopathological and ultrastructural examination.

Results: Alkali exposure induced extensive central corneal scarring, ocular surface irregularity, and neovascularization in one case. All implants showed complete epithelial coverage by four weeks post-operative, but with accompanying suture-induced vascularization in 6/12 cases. A stable, stratified epithelium with hemidesmosomal adhesion complexes regenerated over all implants, and subbasal nerve regeneration was observed in allograft and RHCIII-MPC implants. Initially acellular biosynthetic implants were populated with host-derived keratocytes as stromal haze subsided and stromal collagen was remodeled. Notably, RHCIII-MPC implants exhibited resistance to vascular ingrowth while supporting endogenous cell and nerve repopulation.

Conclusion: Biosynthetic implants based on RHC promoted cell and nerve repopulation in alkali burned rabbit eyes. In RHCIII-MPC implants, evidence of an enhanced resistance to neovascularization was additionally noted.

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Research in Vision and Opthalmology , 2011. Vol. 52, nr 2, s. 651-657
Nationell ämneskategori
Medicin och hälsovetenskap
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URN: urn:nbn:se:liu:diva-65890DOI: 10.1167/iovs.10-5224ISI: 000287846300002OAI: oai:DiVA.org:liu-65890DiVA, id: diva2:399903
Anmärkning
Original Publication: Joanne M. Hackett, Neil Lagali, Kimberley Merrett, Henry Edelhauser, Yifei Sun, Lisha Gan, May Griffith and Per Fagerholm, Biosynthetic corneal implants for replacement of pathologic corneal tissue: performance in a controlled rabbit alkali burn model, 2011, Investigative Ophthalmology and Visual Science. http://dx.doi.org/10.1167/iovs.10-5224 Copyright: Research in Vision and Opthalmology http://www.arvo.org/Tillgänglig från: 2011-02-24 Skapad: 2011-02-24 Senast uppdaterad: 2018-01-22

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Hackett, Joanne M.Lagali, NeilGan, LishaGriffith, MayFagerholm, Per

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Hackett, Joanne M.Lagali, NeilGan, LishaGriffith, MayFagerholm, Per
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HälsouniversitetetOftalmiatrikÖgonkliniken US/LiMInstitutionen för klinisk och experimentell medicin
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