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Functionalisation of recombinant spider silk with conjugated polyelectrolytes
Linköpings universitet, Institutionen för fysik, kemi och biologi, Biomolekylär och Organisk Elektronik. Linköpings universitet, Tekniska högskolan.
Department of Anatomy, Physiology and Biochemistry, SLU, Biomedical Centre, Uppsala, Sweden.
Linköpings universitet, Institutionen för fysik, kemi och biologi, Biomolekylär och Organisk Elektronik. Linköpings universitet, Tekniska högskolan.
Department of Molecular Biology, Uppsala BioCenter, SLU, Biomedical Centre, Uppsala, Sweden .
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2011 (Engelska)Ingår i: Journal of Materials Chemistry, ISSN 0959-9428, E-ISSN 1364-5501, Vol. 21, nr 9, s. 2909-2915Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Conjugated polyelectrolytes are demonstrated to permit facile staining of recombinant spider silk fibres. We find that the polyelectrolyte concentration and pH of the staining solution as well as the incubation temperature strongly influence the efficiency of this self-assembly process, which appears to be principally mediated through favourable electrostatic interactions. Thus, depending on the choice of staining conditions as well as the polyelectrolyte, electrically conductive or photoluminescent recombinant silk fibres could be produced. In addition, staining of natural Bombyx mori silk is established, which emphasises the versatility of the here advanced approach to functionalise silk-based materials.

Ort, förlag, år, upplaga, sidor
Royal Society of Chemistry, 2011. Vol. 21, nr 9, s. 2909-2915
Nationell ämneskategori
Teknik och teknologier
Identifikatorer
URN: urn:nbn:se:liu:diva-66131DOI: 10.1039/c0jm03270kISI: 000287369300019OAI: oai:DiVA.org:liu-66131DiVA, id: diva2:401952
Tillgänglig från: 2011-03-04 Skapad: 2011-03-04 Senast uppdaterad: 2017-12-11
Ingår i avhandling
1. On decoration of biomolecular scaffolds with a conjugated polyelectrolyte
Öppna denna publikation i ny flik eller fönster >>On decoration of biomolecular scaffolds with a conjugated polyelectrolyte
2017 (Engelska)Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
Abstract [en]

Biotemplating is the art of using a biological structure as a scaffold which is decorated with a functional material. In this fashion the structures will gain new functionalities and biotemplating offers a simple route of mass-producing mesoscopic material with new interesting properties. Biological structures are abundant and come in a great variety of elaborate and due to their natural origin they could be more suitable for interaction with biological systems than wholly synthetic materials. Conducting polymers are a novel class of material which was developed just 40 years ago and are well suited for interaction with biological material due to their organic composition. Furthermore the electronic properties of the conducting polymers can be tuned giving rise to dynamic control of the behavior of the material. Self-assembly processes are interesting since they do not require complicated or energy demanding processing conditions. This is particularly important as most biological materials are unstable at elevated temperatures or harsh environments. The main aim of this thesis is to show the possibility of using self-assembly to decorate a conducting polymer onto various biotemplates. Due to the intrinsic variety in charge, size and structure between the available natural scaffolds it is difficult, if not impossible, to find a universal method.

In this thesis we show how biotemplating can be used to create new hybrid materials by self-assembling a conducting polymer with biological structures based on DNA, protein, lipids and cellulose, and in this fashion create material with novel optical and electronic properties.

Ort, förlag, år, upplaga, sidor
Linköping: Linköping University Electronic Press, 2017. s. 51
Serie
Linköping Studies in Science and Technology. Dissertations, ISSN 0345-7524 ; 1885
Nationell ämneskategori
Polymerkemi
Identifikatorer
urn:nbn:se:liu:diva-141675 (URN)10.3384/diss.diva-141675 (DOI)9789176854372 (ISBN)
Disputation
2017-10-19, Planck, Fysikhuset, Campus Valla, Linköping, 10:15 (Engelska)
Opponent
Handledare
Forskningsfinansiär
Stiftelsen för strategisk forskning (SSF)Knut och Alice Wallenbergs Stiftelse
Tillgänglig från: 2017-10-04 Skapad: 2017-10-04 Senast uppdaterad: 2019-10-11Bibliografiskt granskad

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Müller, ChristianElfwing, AndersKarlsson, RogerHamedi, MahiarInganäs, Olle

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