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Initial development towards in vitro toxicity assay for lactate dehydrogenase using surface plasmon resonance
Linköpings universitet, Institutionen för fysik, kemi och biologi, Teknisk biologi. Linköpings universitet, Tekniska högskolan.
Linköpings universitet, Institutionen för fysik, kemi och biologi, Teknisk biologi. Linköpings universitet, Tekniska högskolan.ORCID-id: 0000-0001-9711-794X
(engelsk)Manuskript (preprint) (Annet vitenskapelig)
Abstract [en]

Work with initial development towards in vitro toxicity assay for lactate dehydrogenase (LDH) using surface plasmon resonance is described. LDH is one of the important versatile biomarkers in in vitro hepatotoxicity. In this report LDH is detected by surface plasmon resonance using antibodies directly immobilized to the sensor surface. The assay is further extended with protein G immobilized to the surface to capture the antibody ligand. This has the advantage of simultaneously and on site purify and orient antibody ligand.

Emneord [en]
Lactate dehydrogenase, surface plasmon resonance, in vitro toxicity, biosensor, protein G
HSV kategori
Identifikatorer
URN: urn:nbn:se:liu:diva-73409OAI: oai:DiVA.org:liu-73409DiVA, id: diva2:471881
Tilgjengelig fra: 2012-01-03 Laget: 2012-01-03 Sist oppdatert: 2019-01-22bibliografisk kontrollert
Inngår i avhandling
1. Exploring the SPR methodology for monitoring of critical attributes in toxicity testing and bioproduction
Åpne denne publikasjonen i ny fane eller vindu >>Exploring the SPR methodology for monitoring of critical attributes in toxicity testing and bioproduction
2012 (engelsk)Licentiatavhandling, med artikler (Annet vitenskapelig)
Abstract [en]

Analysis of biological components is central in bioprocess monitoring, process control, product quality control and cell based toxicity assaying. One of these themes that is pursued in this thesis is the use of biosensors for monitoring of molecular markers, exploiting the natural selectivity of biomolecules. Another is the use of glycoconjugates to monitor the activity of biomolecules in a flu vaccine process is studied and were the sensor is based on the concept of weak affinity giving fast response time for the sensor.

A third theme is monitoring of cell cultures used for toxicity testing different protein markers is of interest.

When developing biosensor surfaces for new antigens commercial preparations of antibodies are often used. In this work we have chosen to look at lactate dehydrogenase (LDH) and describe the preparation and characterisation of antibody used in biosensor surface development.

The design of a sensor surface is important for the characteristics of a sensor. By binding antibodies in an oriented manner to the surface a better control of the properties of the antibodies is achieved. The demonstrated method also has the advantage of in situ purification and provides a flexible platform for antibody evaluation and sensor development.

In one sentence this thesis explores the possibility of utilizing recognition elements of a biosensor surface. In particular, surface plasmon resonance (SPR) is used as the primary biosensing tool, however most findings in are relevant for other biosensors.

Moreover, the thesis approaches existing bioanalytical impediments, such as purity and accessibility of the recognition elements on the sensor surface and preparation strategies to achieve this.

sted, utgiver, år, opplag, sider
Linköping: Linköping University Electronic Press, 2012. s. 19
Serie
Linköping Studies in Science and Technology. Thesis, ISSN 0280-7971 ; 1517
HSV kategori
Identifikatorer
urn:nbn:se:liu:diva-73410 (URN)LIU-TEK-LIC-2012:2 (Lokal ID)978-91-7519-971-9 (ISBN)LIU-TEK-LIC-2012:2 (Arkivnummer)LIU-TEK-LIC-2012:2 (OAI)
Presentation
2012-01-31, Panck, Fysikhuset, Campus Valla, Linköpings Universitet, Linköping, 09:15 (svensk)
Opponent
Veileder
Tilgjengelig fra: 2012-01-03 Laget: 2012-01-03 Sist oppdatert: 2019-12-19bibliografisk kontrollert

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