liu.seSearch for publications in DiVA
Endre søk
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Coupling of α1-adrenoceptors to ERK1/2 in the human prostate
University of Munich, Germany.
University of Munich, Germany.
University of Munich, Germany.
University of Munich, Germany.
Vise andre og tillknytning
2011 (engelsk)Inngår i: Urologia internationalis, ISSN 0042-1138, E-ISSN 1423-0399, Vol. 86, nr 4, s. 427-433Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

INTRODUCTION: α1-Adrenoceptors are considered critical for the regulation of prostatic smooth muscle tone. However, previous studies suggested further α1-adrenoceptor functions besides contraction. Here, we investigated whether α1-adrenoceptors in the human prostate may activate extracellular signal-regulated kinases (ERK1/2).

METHODS: Prostate tissues from patients undergoing radical prostatectomy were stimulated in vitro. Activation of ERK1/2 was assessed by Western blot analysis. Expression of ERK1/2 was studied by immunohistochemistry. The effect of ERK1/2 inhibition by U0126 on phenylephrine-induced contraction was studied in organ-bath experiments.

RESULTS: Stimulation of human prostate tissue with noradrenaline (30 μM) or phenylephrine (10 μM) resulted in ERK activation. This was reflected by increased levels of phosphorylated ERK1/2. Expression of ERK1/2 in the prostate was observed in smooth muscle cells. Incubation of prostate tissue with U0126 (30 μM) resulted in ERK1/2 inhibition. Dose-dependent phenylephrine-induced contraction of prostate tissue was not modulated by U0126.

CONCLUSIONS: α1-Adrenoceptors in the human prostate are coupled to ERK1/2. This may partially explain previous observations suggesting a role of α1-adrenoceptors in the regulation of prostate growth.

sted, utgiver, år, opplag, sider
2011. Vol. 86, nr 4, s. 427-433
Emneord [en]
Prostate hyperplasia, 1-Adrenoceptor, Extracellular signal-regulated kinase, Smooth muscle, Lower urinary tract symptoms, α1-Adrenoceptor blocker
HSV kategori
Identifikatorer
URN: urn:nbn:se:liu:diva-77365DOI: 10.1159/000322639PubMedID: 21293101OAI: oai:DiVA.org:liu-77365DiVA, id: diva2:526549
Tilgjengelig fra: 2012-05-14 Laget: 2012-05-14 Sist oppdatert: 2017-12-07

Open Access i DiVA

Fulltekst mangler i DiVA

Andre lenker

Forlagets fulltekstPubMed

Personposter BETA

Hedlund, Petter

Søk i DiVA

Av forfatter/redaktør
Hedlund, Petter
Av organisasjonen
I samme tidsskrift
Urologia internationalis

Søk utenfor DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric

doi
pubmed
urn-nbn
Totalt: 109 treff
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf