liu.seSearch for publications in DiVA
Endre søk
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Tracking a complete voltage-sensor cycle with metal-ion bridges
Linköpings universitet, Hälsouniversitetet. Linköpings universitet, Institutionen för klinisk och experimentell medicin, Avdelningen för cellbiologi.
Linköpings universitet, Institutionen för klinisk och experimentell medicin, Cellbiologi. Linköpings universitet, Hälsouniversitetet.
Linköpings universitet, Institutionen för klinisk och experimentell medicin, Cellbiologi. Linköpings universitet, Hälsouniversitetet.ORCID-id: 0000-0001-8493-0114
Linköpings universitet, Institutionen för klinisk och experimentell medicin, Cellbiologi. Linköpings universitet, Hälsouniversitetet.
Vise andre og tillknytning
2012 (engelsk)Inngår i: Proceedings of the National Academy of Sciences of the United States of America, ISSN 0027-8424, E-ISSN 1091-6490, Vol. 109, nr 22, s. 8552-8557Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Voltage-gated ion channels open and close in response to changes in membrane potential, thereby enabling electrical signaling in excitable cells. The voltage sensitivity is conferred through four voltage-sensor domains (VSDs) where positively charged residues in the fourth transmembrane segment (S4) sense the potential. While an open state is known from the Kv1.2/2.1 X-ray structure, the conformational changes underlying voltage sensing have not been resolved. We present 20 additional interactions in one open and four different closed conformations based on metal-ion bridges between all four segments of the VSD in the voltage-gated Shaker K channel. A subset of the experimental constraints was used to generate Rosetta models of the conformations that were subjected to molecular simulation and tested against the remaining constraints. This achieves a detailed model of intermediate conformations during VSD gating. The results provide molecular insight into the transition, suggesting that S4 slides at least 12 angstrom along its axis to open the channel with a 3(10) helix region present that moves in sequence in S4 in order to occupy the same position in space opposite F290 from open through the three first closed states.

sted, utgiver, år, opplag, sider
National Academy of Sciences , 2012. Vol. 109, nr 22, s. 8552-8557
Emneord [en]
electrophysiology, inactivation, Xenopus oocytes, voltage clamp, conformational transition
HSV kategori
Identifikatorer
URN: urn:nbn:se:liu:diva-78812DOI: 10.1073/pnas.1116938109ISI: 000304881700044OAI: oai:DiVA.org:liu-78812DiVA, id: diva2:536044
Merknad

Funding Agencies|Swedish Research Council||Swedish Heart-Lung Foundation||Swedish Brain Foundation||County Council of Ostergotland||Queen Silvias Anniversary Foundation||King Gustaf V and Queen Victorias Freemasons Foundation||Stina and Birger Johanssons Foundation||Swedish Society for Medical Research||Swedish Foundation for Strategic Research||European Research Council||

Tilgjengelig fra: 2012-06-21 Laget: 2012-06-21 Sist oppdatert: 2018-04-09
Inngår i avhandling
1. Conformational Changes during Potassium-Channel Gating
Åpne denne publikasjonen i ny fane eller vindu >>Conformational Changes during Potassium-Channel Gating
2018 (engelsk)Doktoravhandling, med artikler (Annet vitenskapelig)
Abstract [en]

Voltage-gated ion channels have a paramount importance in many physiological processes such as cell-to-cell communication, action potential-propagation, and cell motility. Voltage-gated ion channels are characterized by their ability to sense membrane voltage and to greatly change channel activity in response to small changes in the voltage. The ability to sense voltage resides in the four voltage-sensor domains (VSDs) surrounding the central ion-conducting pore. Membrane depolarization causes the inside of the membrane to become positively charged, electrostatically repelling the positively charged fourth transmembrane segment (S4), or voltage sensor, in the VSD, causing the voltage sensor to move outwards. This motion provides necessary energy to open the pore and allow ion conductivity. Prolonged channel activation leads to alterations in the selectivity filter which cease ion conductivity, in a process called slow inactivation. In this thesis, we investigated the movement of S4 during activation of the channel. We also studied the communication between the four subunits during activation as well as the communication between the pore domain and VSD during slow inactivation.

We have shown that voltage sensors move approximately 12 Å outwards during activation. The positively charged amino acid residues in S4 create temporary salt bridges with negative counter-charges in the other segments of the VSD as it moves through a membrane. We have also shown that the movement of one of the four voltage sensors can affect the movement of the neighboring voltage sensors. When at least one voltage sensor has moved to an up-position, it stabilizes other voltage sensors in the up-position, increasing the energy required for the voltage sensor to return to the down position.

We have also shown reciprocal communication between the pore domain and the VSDs. Alterations in the VSD or the interface between the pore and the VSD cause changes in the rate of slow inactivation. Likewise, modifications in the pore domain cause changes to the voltage-sensor movement. This indicates communication between the pore and the VSD during slow inactivation.

The information from our work could be used to find new approaches when designing channel-modifying drugs for the treatment of diseases caused by increased neuronal excitability, such as chronic pain and epilepsy.

sted, utgiver, år, opplag, sider
Linköping: Linköping University Electronic Press, 2018. s. 69
Serie
Linköping University Medical Dissertations, ISSN 0345-0082 ; 1614
Emneord
ion-channel, electrophysiology, neuron, Jonkanal, neurologi, elektrofysiologi, neuron, aktionspotential
HSV kategori
Identifikatorer
urn:nbn:se:liu:diva-146967 (URN)10.3384/diss.diva-146967 (DOI)9789176853382 (ISBN)
Disputas
2018-05-09, Berzeliussalen, Campus US, Linköping, 13:00 (engelsk)
Opponent
Veileder
Tilgjengelig fra: 2018-04-09 Laget: 2018-04-09 Sist oppdatert: 2018-04-16bibliografisk kontrollert

Open Access i DiVA

fulltext(730 kB)462 nedlastinger
Filinformasjon
Fil FULLTEXT01.pdfFilstørrelse 730 kBChecksum SHA-512
55427cbadce869b258ab51dea3bf6a80577a6ebcc1f291635a5ec8300e87474ba86188749f4db22601f8329b62325e5926f738e00d4404502f7091e10a7f9528
Type fulltextMimetype application/pdf

Andre lenker

Forlagets fulltekst

Personposter BETA

Henrion, UlrikeRenhorn, JakobBörjesson, SaraWallner, BjörnElinder, Fredrik

Søk i DiVA

Av forfatter/redaktør
Henrion, UlrikeRenhorn, JakobBörjesson, SaraWallner, BjörnElinder, Fredrik
Av organisasjonen
I samme tidsskrift
Proceedings of the National Academy of Sciences of the United States of America

Søk utenfor DiVA

GoogleGoogle Scholar
Totalt: 462 nedlastinger
Antall nedlastinger er summen av alle nedlastinger av alle fulltekster. Det kan for eksempel være tidligere versjoner som er ikke lenger tilgjengelige

doi
urn-nbn

Altmetric

doi
urn-nbn
Totalt: 355 treff
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf