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Soluble plantain fibre blocks adhesion and M-cell translocation of intestinal pathogens
Linköpings universitet, Institutionen för klinisk och experimentell medicin, Kirurgi. Linköpings universitet, Hälsouniversitetet.
University of Liverpool, England .
University of Liverpool, England .
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2013 (Engelska)Ingår i: Journal of Nutritional Biochemistry, ISSN 0955-2863, E-ISSN 1873-4847, Vol. 24, nr 1, s. 97-103Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Dietary fibres may have prebiotic effects mediated by promotion of beneficial bacteria. This study explores the possibility that soluble plant fibre may also improve health by inhibiting epithelial adhesion and translocation by pathogenic bacteria. We have focussed on soluble non-starch polysaccharide (NSP) from plantain bananas (Musa spp.) which previous studies showed to be particularly effective at blocking Escherichia coli epithelial adherence. In vitro and ex vivo studies assessed the ability of plantain NSP to inhibit epithelial cell adhesion and invasion of various bacterial pathogens, and to inhibit their translocation through microfold (M)-cells and human Peyers patches mounted in Ussing chambers. Plantain NSP showed dose-related inhibition of epithelial adhesion and M-cell translocation by a range of pathogens. At 5 mg/ml, a concentration readily achievable in the gut lumen, plantain NSP inhibited adhesion to Caco2 cells by Salmonella Typhimurium (85.0 +/- 8.2%, Pandlt;.01), Shigella sonnei (46.6 +/- 29.3%. Pandlt;.01), enterotoxigenic E.coli (56.1 +/- 23.7%, Pandlt;.05) and Clostridium difficile (67.6 +/- 12.3%, Pandlt;.001), but did not inhibit adhesion by enteropathogenic E.coli. Plantain NSP also inhibited invasion of Caco2 cells by S. Typhimurium (80.2 +/- 9.7%) and Sh. sonnei (46.7 +/- 13.4%); Pandlt;.01. Plantain NSP, 5 mg/ml, also inhibited translocation of S. Typhimurium and Sh. sonnei across M-cells by 73.3 +/- 5.2% and 46.4 +/- 7.7% respectively (Pandlt;.05). Similarly, S. Typhimurium translocation across Peyers patches was reduced 65.9 +/- 8.1% by plantain NSP (Pandlt;.01). Soluble plantain fibre can block epithelial adhesion and M-cell translocation of intestinal pathogens. This represents an important novel mechanism by which soluble dietary fibres can promote intestinal health and prevent infective diarrhoea. Crown Copyright

Ort, förlag, år, upplaga, sidor
Elsevier , 2013. Vol. 24, nr 1, s. 97-103
Nyckelord [en]
Dietary fibre, Diarrhoea, Enteric infections, Peyers patches, M (microfold) cell, Mucosal immunology
Nationell ämneskategori
Medicin och hälsovetenskap
Identifikatorer
URN: urn:nbn:se:liu:diva-87958DOI: 10.1016/j.jnutbio.2012.02.013ISI: 000312479700013OAI: oai:DiVA.org:liu-87958DiVA, id: diva2:601037
Anmärkning

Funding Agencies|University of Liverpool Reach Out Growth Fund award|ROGF-N0306|Biotechnology & Biosciences Research Council|BB/G01969X/1|Liverpool National Institute for Health Research Specialist Biomedical Research Centre for Microbial Diseases|01CD1|Bo and Vera Ax:son Johnsson Foundation||Swedish Research Council||

Tillgänglig från: 2013-01-28 Skapad: 2013-01-28 Senast uppdaterad: 2017-12-06

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Keita, ÅsaSöderholm, Johan D.

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Keita, ÅsaSöderholm, Johan D.
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KirurgiHälsouniversitetetAvdelningen för kliniska vetenskaperKirurgiska kliniken US
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Journal of Nutritional Biochemistry
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