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Functional gene transfer of HIV DNA by an HIV receptor-independent mechanism
Karolinska Institute, Sweden.
Northwestern University Medical School, Chicago, USA.
Karolinska Institute, Sweden.
Karolinska Institute, Sweden.
Vise andre og tillknytning
1999 (engelsk)Inngår i: Journal of Immunology, ISSN 0022-1767, E-ISSN 1550-6606, Vol. 163, nr 2, s. 736-742Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

HIV-1 enters target cells mainly via binding to CD4 and its coreceptors. The presence of HIV-1 in CD4(-) cells suggests, however, that there exist other mechanisms for viral entry, Here it is reported that HIV-1 DNA may be transferred from one cell to another by uptake of apoptotic bodies in a CD4-independent way. This was investigated by coculturing CD4(-), chemokine receptor CCR5(-) and CXCR4(-) human fetal fibroblasts with apoptotic HIV-1-infected HuT78 cells or apoptotic PBMC isolated from HIV-1-infected patients. After 2 wk of coculture, fibroblasts contained HIV-1 DNA and expressed HIV-1 proteins p24 and gp120, Transfer of HIV-1 DNA was verified by coculturing fibroblasts with apoptotic bodies derived from cells infected with a defective HIV-1 virus. These cells contain one integrated copy of a reverse transcriptase (RT)-negative HIV-1 strain (8E5/LAV RT- cells) and consequently cannot produce free virus, Intracellular HIV-1 gag DNA was detected in both fibroblasts and dendritic cells after coculture with apoptotic 8E5/LAV RT- cells. Transfer of viral DNA after uptake of apoptotic bodies mag explain HIV-1 infection of CD4(-) cells in vivo and furthermore may be relevant for Ag presentation.

sted, utgiver, år, opplag, sider
American Association of Immunologists , 1999. Vol. 163, nr 2, s. 736-742
HSV kategori
Identifikatorer
URN: urn:nbn:se:liu:diva-88116ISI: 000081360800027PubMedID: 10395665OAI: oai:DiVA.org:liu-88116DiVA, id: diva2:601813
Tilgjengelig fra: 2013-01-30 Laget: 2013-01-30 Sist oppdatert: 2017-12-06

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