liu.seSök publikationer i DiVA
Ändra sökning
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Melting temperature of surface-tethered DNA
Universitat Rovira i Virgili, Tarragona, Spain.
Universitat Rovira i Virgili, Tarragona, Spain.
Universitat Rovira i Virgili, Tarragona, Spain.ORCID-id: 0000-0001-6889-0351
Universitat Rovira i Virgili, Tarragona, Spain.
2010 (Engelska)Ingår i: Analytical Biochemistry, ISSN 0003-2697, E-ISSN 1096-0309, Vol. 406, nr 1, s. 34-40Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

A method for the accurate determination of the melting temperature (Tm) of surface-immobilized DNAduplexes that exploits the fluorescence-quenching properties of gold is reported. A thiolated singlestrandedDNA probe is chemisorbed onto a gold surface and then hybridized to a fluorophore-labeledcomplementary sequence. On formation of the duplex, the fluorescence of the label is effectivelyquenched by the gold surface. As the temperature is increased and the duplex denatures, the fluorophorelabel moves away from the gold surface and the fluorescence signal is again observed. The increase influorescence is measured as the temperature is ramped, and using first-derivative plots, the Tm is determined.To demonstrate the approach, the Tm of the cystic fibrosis DF508 mutation was determined inthree different phases: in solution, in suspension immobilized on gold nanoparticles, and immobilizedon gold film-coated substrate. The technique was further applied to optimize conditions for differentiationbetween a surface-immobilized DF508 mutant probe and a mutant/wild-type target exploitingincreasing stringency in varying salt and formamide concentrations. The approach has application inoptimization of assay conditions for biosensors that use gold substrates as well as in melting curveanalysis.

Ort, förlag, år, upplaga, sidor
Elsevier, 2010. Vol. 406, nr 1, s. 34-40
Nyckelord [en]
Melting temperature, Surface-immobilized DNA, Fluorescence quenching, Gold nanoparticles
Nationell ämneskategori
Analytisk kemi
Identifikatorer
URN: urn:nbn:se:liu:diva-92076DOI: 10.1016/j.ab.2010.06.049OAI: oai:DiVA.org:liu-92076DiVA, id: diva2:620079
Tillgänglig från: 2013-05-07 Skapad: 2013-05-07 Senast uppdaterad: 2017-12-06Bibliografiskt granskad

Open Access i DiVA

Fulltext saknas i DiVA

Övriga länkar

Förlagets fulltext

Personposter BETA

Beni, Valerio

Sök vidare i DiVA

Av författaren/redaktören
Beni, Valerio
I samma tidskrift
Analytical Biochemistry
Analytisk kemi

Sök vidare utanför DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetricpoäng

doi
urn-nbn
Totalt: 122 träffar
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf