liu.seSök publikationer i DiVA
Ändra sökning
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Differences in the expression of hepatocyte growth factor in acute and chronic bowel inflammation - Implications for diagnosis?
PEAS Institut, Linköping, Sweden.
PEAS Institut, Linköping, Sweden.
Linköpings universitet, Institutionen för klinisk och experimentell medicin, Avdelningen för inflammationsmedicin. Linköpings universitet, Hälsouniversitetet. Östergötlands Läns Landsting, Diagnostikcentrum, Klinisk patologi och klinisk genetik.
Örebro University, Sweden.
Visa övriga samt affilieringar
2013 (Engelska)Ingår i: Advances in Bioscience and Biotechnology, ISSN 2156-8456, E-ISSN 2156-8502, Vol. 4, nr 8A2, s. 33-42Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Background: Hepatocyte growth factor (HGF) acts as an acute phase protein with regenerative properties. HGF is produced systemically and locally during inflammation but exhibits decreased binding affinity to heparan sulphate proteoglycan (HSPG)/glycosaminoglycan during chronic inflammation. We previously observed a high faecal concentration and binding affinity of HGF to HSPG during acute gastroenteritis. High faecal concentrations of calprotectin and HGF have been reported in chronic inflammatory bowel disease (IBD).

Methods: Stool samples from patients with ulcerative colitis in remission (n = 11) or exacerbation (n = 5), microscopic colitis (n = 11), colon cancer (n = 6), or acute gastroenteritis caused by Clostridium difficile (n = 20), as well as healthy controls (n = 7), were analysed for the presence of HGF by ELISA, surface plasmon resonance, SDS-PAGE, and Western blot. Then in two patients with ulcerative colitis exacerbation and C. difficile infection, the expression of HGF and calprotectin was studied in colonic biopsies.

Results: The faecal concentration of HGF was significantly higher in patients with ulcerative colitis compared to the other groups. The binding affinity to dextran was lower in all groups compared to acute inflammation. HGF receptor binding was similar across groups. In a patient with concomitant C. difficile infection and distal ulcerative colitis, HGF was highly expressed in the part of the bowel unaffected by ulcerative colitis, but no expression was found at the site of chronic inflammation. In the patient with total colitis the biopsies showed low expression of HGF. The areas with chronic inflammation exhibited infiltrating calprotectin-stained neutrophils.

Conclusion: HGF is produced locally during inflammation of the bowel. The HGF produced during acute inflammation or exacerbations of chronic inflammation by the unaffected area shows binding affinity to glucosaminoglycans. Measuring HGF binding in faeces and biopsies may be a tool for differentiating between acute and chronic bowel inflammation, which should be assessed thoroughly in future studies.

Ort, förlag, år, upplaga, sidor
Scientific Research Publishing, 2013. Vol. 4, nr 8A2, s. 33-42
Nyckelord [en]
Diarrhoea; Ulcerative Colitis; Dextran Sulphate; HGF; HSPG; Calprotectin
Nationell ämneskategori
Medicin och hälsovetenskap
Identifikatorer
URN: urn:nbn:se:liu:diva-96964DOI: 10.4236/abb.2013.48A2006OAI: oai:DiVA.org:liu-96964DiVA, id: diva2:644791
Tillgänglig från: 2013-09-02 Skapad: 2013-09-02 Senast uppdaterad: 2017-12-06Bibliografiskt granskad

Open Access i DiVA

fulltext(1679 kB)231 nedladdningar
Filinformation
Filnamn FULLTEXT01.pdfFilstorlek 1679 kBChecksumma SHA-512
be265ca2b1fe0d4d6a4bcca12ce3ee6fc28a652820e546cd6905670ba598d0aa90da9daf2dfece9c0e992feac726e1ad80ef1b486d206a6acfd3bd6868e53887
Typ fulltextMimetyp application/pdf

Övriga länkar

Förlagets fulltext

Personposter BETA

Lönn, JohannaOlsson, HansAlmer, Sven

Sök vidare i DiVA

Av författaren/redaktören
Lönn, JohannaOlsson, HansAlmer, Sven
Av organisationen
Avdelningen för inflammationsmedicinHälsouniversitetetKlinisk patologi och klinisk genetikGastroenterologi och hepatologiMagtarmmedicinska klinikenInfektionsmedicinInfektionskliniken i Östergötland
I samma tidskrift
Advances in Bioscience and Biotechnology
Medicin och hälsovetenskap

Sök vidare utanför DiVA

GoogleGoogle Scholar
Totalt: 231 nedladdningar
Antalet nedladdningar är summan av nedladdningar för alla fulltexter. Det kan inkludera t.ex tidigare versioner som nu inte längre är tillgängliga.

doi
urn-nbn

Altmetricpoäng

doi
urn-nbn
Totalt: 208 träffar
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf