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Expression of fatty acid amide hydrolase (FAAH) in human, mouse, and rat urinary bladder and effects of FAAH inhibition on bladder function in awake rats
Munich University, Germany.
San Raffaele University, Milan, Italy.
San Raffaele University, Milan, Italy.
San Raffaele University, Milan, Italy.
Visa övriga samt affilieringar
2012 (Engelska)Ingår i: European Urology, ISSN 0302-2838, E-ISSN 1873-7560, Vol. 61, nr 1, s. 98-106Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

BACKGROUND:

Cannabinoid receptor (CB)-mediated functions may be involved in the regulation of bladder function, but information on endocannabinoid signals during micturition is scarce.

OBJECTIVE:

Investigate the expression of the endocannabinoid-degrading enzyme fatty acid amide hydrolase (FAAH) in human, rat, and mouse bladders and study the effects of inhibition of FAAH during urodynamics in awake rats.

DESIGN, SETTING, AND PARTICIPANTS:

Bladder tissue from humans, mice, and rats was used for measurements. Female Sprague-Dawley rats were administered the FAAH inhibitor oleoyl ethyl amide (OEtA) or vehicle intravenously (IV) or intravesically (IVES) with or without rimonabant (CB1 antagonist) or SR144528 (CB2 antagonist).

MEASUREMENTS:

Real-time transcriptase-polymerase chain reaction, Western blot, immunohistochemistry, and cystometry in awake rats.

RESULTS AND LIMITATIONS:

Messenger RNA and protein for FAAH was expressed in the mucosa of human, mouse, and rat urinary bladders. Immunoreactivities for FAAH and CB2 were codistributed in rat and human urothelium. IV OEtA (0.3mg/kg) to rats increased intercontraction intervals (ICIs), micturition volume (MV), bladder capacity (BC), and threshold pressure (TP) by 17±1%, 16±1%, 17±1%, and 19±5%, respectively (all p<0.05 vs baseline). IVES OEtA (1 and 10mg/l) in rats dose-dependently increased (p<0.05 vs baseline) ICI (19±2% and 35±5%), MV (15±3% and 32±4%), BC (16±2% and 34±4%), and TP (15±1%, 21±3%). SR144528 (IVES 5mg/l) abolished all effects of OEtA, whereas rimonabant only counteracted effects of OEtA on TP.

CONCLUSIONS:

Bladder mucosa of all species expressed FAAH. Rat and human urothelium coexpressed FAAH and CB2. The FAAH inhibitor OEtA altered urodynamic parameters that reflect sensory functions of micturition in rats. Suggesting a role for the endocannabinoid system in bladder mechanoafferent functions of rats, effects of IVES OEtA were abolished by an IVES CB2 antagonist and partly counteracted by an IVES CB1 antagonist.

 

 

Ort, förlag, år, upplaga, sidor
Elsevier, 2012. Vol. 61, nr 1, s. 98-106
Nyckelord [en]
Endocannabinoid Enzyme mRNA Protein CB2 CB1 Mucosa Intravesical Cystometry
Nationell ämneskategori
Medicin och hälsovetenskap
Identifikatorer
URN: urn:nbn:se:liu:diva-100520DOI: 10.1016/j.eururo.2011.09.002ISI: 000297861800020PubMedID: 21930339OAI: oai:DiVA.org:liu-100520DiVA, id: diva2:662818
Tillgänglig från: 2013-11-08 Skapad: 2013-11-08 Senast uppdaterad: 2017-06-27Bibliografiskt granskad

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Hedlund, Petter

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