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Rapid confocal imaging for measuring sound-induced motion of the hearing organ in the apical region
Karolinska Institutet / Karolinska University Hospital, Stockholm, Sweden.
Karolinska Institutet / Karolinska University Hospital, Stockholm, Sweden.
Karolinska Institutet / Karolinska University Hospital, Stockholm, Sweden.
Karolinska Institutet / Karolinska University Hospital, Stockholm, Sweden.
Vise andre og tillknytning
2007 (engelsk)Inngår i: Journal of Biomedical Optics, ISSN 1083-3668, E-ISSN 1560-2281, Vol. 12, nr 2, s. 021005-1-021005-6Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

We describe a novel confocal image acquisition system capable of measuring the sound-evoked motion of the organ of Corti. The hearing organ is imaged with a standard laser scanning confocal microscope during sound stimulation. The exact temporal relation between each image pixel and the sound stimulus is quantified. The motion of the structures under study is obtained by fitting a Fourier series to the time dimension of a continuous sequence of acquired images. Previous versions of this acquisition system used a simple search to find pixels with similar phase values. The Fourier series approach permits substantially faster image acquisition with reduced noise levels and improved motion estimation. The system is validated by imaging various vibrating samples attached to a feedback-controlled piezoelectric translator. When using a rigid sample attached to the translator, the system is capable of measuring motion with peak-to-peak amplitudes smaller than 50 nm with an error below 20% at frequencies between 50 and 600 Hz. Examples of image sequences from the inner ear are given, along with detailed performance characteristics of the method.

sted, utgiver, år, opplag, sider
SPIE - International Society for Optical Engineering, 2007. Vol. 12, nr 2, s. 021005-1-021005-6
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URN: urn:nbn:se:liu:diva-101066DOI: 10.1117/1.2718568PubMedID: 17477712OAI: oai:DiVA.org:liu-101066DiVA, id: diva2:665151
Tilgjengelig fra: 2013-11-19 Laget: 2013-11-19 Sist oppdatert: 2018-01-11bibliografisk kontrollert

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Fridberger, Anders

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