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Isolation and culture of endothelial cells, pericytes and perivascular resident macrophage-like melanocytes from the young mouse ear
Oregon Health & Science University, Portland, USA.
Oregon Health & Science University, Portland, USA.
Lawrence Berkeley National Laboratory, California, USA.
Lawrence Berkeley National Laboratory, California, USA.
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2013 (engelsk)Inngår i: Nature Protocols, ISSN 1754-2189, E-ISSN 1750-2799, Vol. 8, nr 4, s. 709-720Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

This protocol describes a growth medium-based approach for obtaining cochlear endothelial cells (ECs), pericytes (PCs) and perivascular resident macrophage-like melanocytes (PVM/Ms) from the stria vascularis of mice aged between P10 and P15 (P, postnatal day). The procedure does not involve mechanical or enzymatic digestion of the sample tissue. Explants of stria vascularis, 'mini-chips', are selectively cultured in growth medium, and primary cell lines are obtained in 7-10 d. The method is simple and reliable, and it provides high-quality ECs, PVM/Ms and PCs with a purity >90% after two passages. This protocol is suitable for producing primary culture cells from organs and tissues of small volume and high anatomical complexity, such as the inner ear capillaries. The highly purified primary cell lines enable cell culture-based in vitro modeling of cell-cell interactions, barrier control function and drug action.

sted, utgiver, år, opplag, sider
Nature Publishing Group, 2013. Vol. 8, nr 4, s. 709-720
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URN: urn:nbn:se:liu:diva-101045DOI: 10.1038/nprot.2013.033PubMedID: 23493068OAI: oai:DiVA.org:liu-101045DiVA, id: diva2:665175
Tilgjengelig fra: 2013-11-19 Laget: 2013-11-19 Sist oppdatert: 2018-01-11bibliografisk kontrollert

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