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Glutaredoxin regulates vascular development by reversible glutathionylation of sirtuin 1
Karolinska Institute, Sweden .
Linköpings universitet, Institutionen för medicin och hälsa, Avdelningen för kardiovaskulär medicin. Linköpings universitet, Hälsouniversitetet. Karolinska Institute, Sweden .
University of Dusseldorf, Germany .
Karolinska Institute, Sweden .
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2013 (Engelska)Ingår i: Proceedings of the National Academy of Sciences of the United States of America, ISSN 0027-8424, E-ISSN 1091-6490, Vol. 110, nr 50, s. 20057-20062Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Embryonic development depends on complex and precisely orchestrated signaling pathways including specific reduction/oxidation cascades. Oxidoreductases of the thioredoxin family are key players conveying redox signals through reversible posttranslational modifications of protein thiols. The importance of this protein family during embryogenesis has recently been exemplified for glutaredoxin 2, a vertebrate-specific glutathione-disulfide oxidoreductase with a critical role for embryonic brain development. Here, we discovered an essential function of glutaredoxin 2 during vascular development. Confocal microscopy and time-lapse studies based on two-photon microscopy revealed that morpholino-based knockdown of glutaredoxin 2 in zebrafish, a model organism to study vertebrate embryogenesis, resulted in a delayed and disordered blood vessel network. We were able to show that formation of a functional vascular system requires glutaredoxin 2-dependent reversible S-glutathionylation of the NAD(+)-dependent protein deacetylase sirtuin 1. Using mass spectrometry, we identified a cysteine residue in the conserved catalytic region of sirtuin 1 as target for glutaredoxin 2-specific deglutathionylation. Thereby, glutaredoxin 2-mediated redox regulation controls enzymatic activity of sirtuin 1, a mechanism we found to be conserved between zebrafish and humans. These results link S-glutathionylation to vertebrate development and successful embryonic angiogenesis.

Ort, förlag, år, upplaga, sidor
National Academy of Sciences , 2013. Vol. 110, nr 50, s. 20057-20062
Nyckelord [en]
proteomics, cardiovascular system
Nationell ämneskategori
Naturvetenskap
Identifikatorer
URN: urn:nbn:se:liu:diva-102769DOI: 10.1073/pnas.1313753110ISI: 000328061700035OAI: oai:DiVA.org:liu-102769DiVA, id: diva2:683928
Anmärkning

Funding Agencies|Karolinska Institutet|2379/07-225|Heinrich Heine University research commission of the Medical Faculty||Graduate School iBrain||CLICK imaging facility||KAW|2006.0192|Swedish Cancer Society|961|Swedish Research Council|K2012-68X-03529-41-3|

Tillgänglig från: 2014-01-07 Skapad: 2013-12-26 Senast uppdaterad: 2017-12-06

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Dahl Ejby Jensen, LasseCao, Yihai

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