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Selective Detachment of Human Primary Keratinocytes and Fibroblasts Using an Addressable Conjugated Polymer Matrix
Linköpings universitet, Institutionen för teknik och naturvetenskap, Fysik och elektroteknik. Linköpings universitet, Tekniska högskolan.
Linköpings universitet, Institutionen för klinisk och experimentell medicin, Avdelningen för kliniska vetenskaper. Linköpings universitet, Hälsouniversitetet.
Linköpings universitet, Institutionen för teknik och naturvetenskap, Fysik och elektroteknik. Linköpings universitet, Tekniska högskolan.
Linköpings universitet, Institutionen för fysik, kemi och biologi. Linköpings universitet, Tekniska högskolan.
Visa övriga samt affilieringar
2014 (Engelska)Manuskript (preprint) (Övrigt vetenskapligt)
Abstract [en]

Conjugated polymers have been used in several applications for electronic control of cell cultures over the last years. We have shown detachment of human endothelial cells using a thin film of a self-doped water-soluble conjugated polymer. Upon electrochemical oxidation, the film swells, cracks and finally detaches taking cells cultured on top along with it. The polymer can be patterned using standard photolithography. The detachment only occurs above a threshold potential of +0.7 V and this fact has been used to create a simple actively addressed matrix, based on a resistor network placed in an encapsulated back plane. The matrix has individually detachable pixels. In this paper we have evaluated detachment of human primary keratinocytes and fibroblasts using PEDOT-S:H. In addition, we have studied effects of serum proteins, added as nutrients to the cell culture medium, on the detachment properties. It was found that at prolonged incubation times protein adhesion effectively stopped the detachment. Using shorter incubation times before detachment, both keratinocytes and fibroblasts can be detached using a regular planar device as well as the matrix device for selective detachment. Spatial control of detachment could be of use when selecting cells for clonal expansion and in order to obtain a homogeneous starting population of cells aimed for tissue engineering purposes.

Ort, förlag, år, upplaga, sidor
2014.
Nationell ämneskategori
Polymerkemi Cellbiologi
Identifikatorer
URN: urn:nbn:se:liu:diva-106252OAI: oai:DiVA.org:liu-106252DiVA, id: diva2:715038
Tillgänglig från: 2014-04-30 Skapad: 2014-04-30 Senast uppdaterad: 2017-02-03Bibliografiskt granskad
Ingår i avhandling
1. Electronic Control of Cell Cultures Using Conjugated Polymer Surfaces
Öppna denna publikation i ny flik eller fönster >>Electronic Control of Cell Cultures Using Conjugated Polymer Surfaces
2014 (Engelska)Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
Abstract [en]

In the field of bioelectronics various electronic materials and devices are used in combination with biological systems in order to create novel applications within cell biology and medicine. A famous example of a successful bioelectronics application is the pacemaker. Metals are the most common electrical conductors, whereas polymers are generally considered being insulators. However, in the late 1970s it was shown that a special class of polymers with conjugated double bonds, could in fact, after some chemical modifications, conduct electricity. This was the start of the research field known as conducting polymers, and then later on organic electronics, a research area that has grown rapidly during the last decades. Conjugated polymers are also suitable to interact and interface with cells and tissues, as they are generally soft, flexible and biocompatible. In addition, their chemical properties can be tailor-made through synthesis to fit biological requirements and functions. During the last years applications using organic bioelectronics have become numerous.

This thesis describes applications based on different conjugated polymers aiming to stimulate and control cell cultures. When culturing cells it is of interest to be able to control events such as adhesion, spreading, proliferation, differentiation and detachment. First, the impact of different polymer compositions and redox states on the adhesion of bacteria and subsequent biofilm formation was investigated. Similar polymer electrodes were also used to steer differentiation of neural stem cells, through changes in the surface exposure of a relevant biomolecule. Controlled delivery of molecules was achieved by coating nanoporous membranes with polymers that swell and contract when changing the redox state. Finally, electronic control over cell detachment using a water-soluble polymer was achieved. When applying a positive potential to this polymer, it swells, cracks and finally detaches, taking the cells that was cultured on top along with it. Together, the work and results presented in this thesis demonstrate a versatile conjugated polymer technology to achieve electronic control of the different growth stages of cell cultures as well as cellular functions.

Ort, förlag, år, upplaga, sidor
Linköping: Linköping University Electronic Press, 2014. s. 64
Serie
Linköping Studies in Science and Technology. Dissertations, ISSN 0345-7524 ; 1594
Nationell ämneskategori
Naturvetenskap
Identifikatorer
urn:nbn:se:liu:diva-106254 (URN)10.3384/diss.diva-106254 (DOI)978-91-7519-340-3 (ISBN)
Disputation
2014-05-23, K2, Kåkenhus, Campus Valla, Linköpings universitet, Linköping, 10:15 (Engelska)
Opponent
Handledare
Tillgänglig från: 2014-04-30 Skapad: 2014-04-30 Senast uppdaterad: 2019-11-19Bibliografiskt granskad

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Persson, Kristin MLönnqvist, Susanna LönnqvistTybrandt, KlasGabrielsson, RogerKratz, GunnarBerggren, Magnus

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Persson, Kristin MLönnqvist, Susanna LönnqvistTybrandt, KlasGabrielsson, RogerKratz, GunnarBerggren, Magnus
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Fysik och elektroteknikTekniska högskolanAvdelningen för kliniska vetenskaperHälsouniversitetetInstitutionen för fysik, kemi och biologiHand- och plastikkirurgiska kliniken US
PolymerkemiCellbiologi

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