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A highly UV-transparent fused silica biochip for sensitive hepatotoxicity testing by autofluorescence
Linköpings universitet, Institutionen för fysik, kemi och biologi, Teknisk biologi. Linköpings universitet, Tekniska högskolan.
University of Gothenburg, Sweden .
Lund University, Sweden .
Linköpings universitet, Institutionen för fysik, kemi och biologi, Teknisk biologi. Linköpings universitet, Tekniska högskolan.ORCID-id: 0000-0001-9711-794X
2014 (engelsk)Inngår i: BioChip Journal, ISSN 1976-0280, Vol. 8, nr 2, s. 115-121Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Fabrication and application of a non-fluorescent and UV-transparent microfluidic biochip in fused silica that allows sensitive autofluorescence detection are described. The biochip is particularly useful in cell-based assays where the most informative autofluorescence signals from the cells reside in the ultraviolet spectral range and where plastic labware materials commonly used in cell culture work severely disturb such measurements. In this study the fused silica biochip was used for measuring intrinsic autofluorescence from liver cells in order to assess hepatotoxic effects of drugs. The assessment assay was carried out with the human liver cell line HepG2 under perfusion conditions in the microfluidics of the biochip. The autofluorescence from the.liver cells exposed to quinidine was readily recorded without background disturbance and correlated well with reference toxicity data.

sted, utgiver, år, opplag, sider
Springer, 2014. Vol. 8, nr 2, s. 115-121
Emneord [en]
Autofluorescence; Drug testing; Microfluidic chip; Fused silica; In vitro toxicity; HepG2 cell line
HSV kategori
Identifikatorer
URN: urn:nbn:se:liu:diva-109130DOI: 10.1007/s13206-014-8206-zISI: 000337717900006OAI: oai:DiVA.org:liu-109130DiVA, id: diva2:737527
Tilgjengelig fra: 2014-08-13 Laget: 2014-08-11 Sist oppdatert: 2019-01-22bibliografisk kontrollert

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