liu.seSearch for publications in DiVA
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Chemistry-specific surface adsorption of the barnacle settlement-inducing protein complex
Linköping University, Department of Physics, Chemistry and Biology, Molecular Physics. Linköping University, Faculty of Science & Engineering.
Newcastle University, England.
Newcastle University, England.
Linköping University, Department of Physics, Chemistry and Biology, Molecular Physics. Linköping University, The Institute of Technology.
Show others and affiliations
2015 (English)In: Interface Focus, ISSN 2042-8898, E-ISSN 2042-8901, Vol. 5, no 1, p. 20140047-Article in journal (Refereed) Published
Abstract [en]

Gregarious settlement in barnacle larvae (cyprids) is induced by a contact pheromone, the settlement-inducing protein complex (SIPC). The SIPC has been identified both in the cuticle of adult barnacles and in the temporary adhesive secretion (footprint) of cyprids. Besides acting as a settlement inducer, the presence of the SIPC in footprints points to its additional involvement in the adhesion process. SIPC adsorption behaviour was therefore investigated on a series of self-assembled monolayers (SAMs) by surface plasmon resonance at the pH of seawater (8.3). Fibrinogen and alpha(2)-macroglobulin (A2M) (blood complement protease inhibitors with which the SIPC shares 29% sequence homology) were used in the adsorption experiments as positive and negative standards, respectively. The mass uptake of the SIPC was comparable to that of fibrinogen, with adsorption observed even on the protein-resistant oligo(ethylene glycol) surface. Notably, on the positively charged SAM the SIPC showed a kinetic overshoot, indicating a metastable configuration causing the amount of adsorbed protein to temporarily exceed its equilibrium value. A2M adsorption was low or negligible on all SAMs tested, except for the positively charged surface, indicating that A2M adsorption is mainly driven by electrostatics. Evaluation of SIPC non-specific adsorption kinetics revealed that it adsorbed irreversibly and non-cooperatively on all surfaces tested.

Place, publisher, year, edition, pages
Royal Society, The , 2015. Vol. 5, no 1, p. 20140047-
Keywords [en]
barnacle; self-assembled monolayer; surface plasmon resonance; adhesion; settlement-inducing protein complex; kinetic overshoot
National Category
Physical Sciences
Identifiers
URN: urn:nbn:se:liu:diva-113714DOI: 10.1098/rsfs.2014.0047ISI: 000346703200003OAI: oai:DiVA.org:liu-113714DiVA, id: diva2:784675
Note

Funding Agencies|European Community [237997]; Office of Naval Research [N00014-08-1-1240]; COST Action [TD0906]

Available from: 2015-01-30 Created: 2015-01-29 Last updated: 2017-12-05

Open Access in DiVA

fulltext(1575 kB)325 downloads
File information
File name FULLTEXT02.pdfFile size 1575 kBChecksum SHA-512
3781bb3aae04a930a372f6db63e4d346b7142f53325eafbe102a5e81898a72cbc57153e553564a820f28ac7a5659d1d1dc8870642e8e8cad050c2b24cfb61a44
Type fulltextMimetype application/pdf

Other links

Publisher's full text

Authority records

Petrone, LuigiEnander, KarinEderth, Thomas

Search in DiVA

By author/editor
Petrone, LuigiEnander, KarinEderth, Thomas
By organisation
Molecular PhysicsFaculty of Science & EngineeringThe Institute of Technology
In the same journal
Interface Focus
Physical Sciences

Search outside of DiVA

GoogleGoogle Scholar
Total: 325 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 414 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf