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Fructose 3-phosphate and 5-phosphoribosyl-1-pyrophosphate formation in perfused human erythrocytes: 31P NMR studies
Linköpings universitet, Institutionen för medicin och hälsa, Avdelningen för radiologiska vetenskaper. Linköpings universitet, Hälsouniversitetet. Linköpings universitet, Centrum för medicinsk bildvetenskap och visualisering, CMIV. Region Östergötland, Centrum för kirurgi, ortopedi och cancervård, Radiofysikavdelningen US. Department of Biochemistry, University of Sydney, Australia..ORCID-id: 0000-0001-8661-2232
Department of Biochemistry, University of Sydney, Australia..
Department of Biochemistry, University of Sydney, Australia..
1994 (engelsk)Inngår i: Magnetic Resonance in Medicine, ISSN 0740-3194, E-ISSN 1522-2594, Vol. 31, nr 2, s. 110-121Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

31P NMR was used to study the formation of fructose 3-phos-phate (F3P) and 5-phosphoribosyl-1-pyrophosphate (PRPP) in perfused human erythrocytes, in the presence of 10 different combinations and concentrations of glucose, inosine, pyru-vate, fructose, and inorganic phosphate (Pi). (1) The cells were immobilized in alginate-coated agarose threads and perfused with a medium containing fructose, and the level of F3P increased continuously over more than 10 h. The net rate of F3P formation was independent of the concentration of 2,3-bis-phosphoglycerate (2,3-DPG) present in the cells. (2) PRPP was formed in high concentrations, relative to normal, in immobilized cells when they were perfused with a medium containing Pi at a low pH (6.6). (3) The 2,3-DPG level decreased simultaneously when the sample was perfused with a medium containing fructose, but without inosine or pyruvate. The measured intracellular pH and free Mg2+ concentration were constant in these experiments. (4) The experiments confirmed the presence of fructose-3-phosphokinase (E.C. 2.7.1.-) and ribose-phosphate pyrophosphokinase (E.C. 2.7.6.1) activity in the human erythrocytes and that the biosynthetic pathways are active in immobilized cells at 37°C. (5) The rates of accumulation of 2,3-DPG and phosphomonoesters (PME) appeared to be strongly correlated.

sted, utgiver, år, opplag, sider
John Wiley & Sons, 1994. Vol. 31, nr 2, s. 110-121
Emneord [en]
cell immobilization;perfused human erythrocytes;fructose 3-phosphate;5-phosphoribosyl-1-pyrophos-phate
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Identifikatorer
URN: urn:nbn:se:liu:diva-114160DOI: 10.1002/mrm.1910310204PubMedID: 7510819OAI: oai:DiVA.org:liu-114160DiVA, id: diva2:790721
Merknad

0740-3194 (Print) Journal Article Research Support, Non-U.S. Gov't

Tilgjengelig fra: 2015-02-25 Laget: 2015-02-11 Sist oppdatert: 2017-12-04bibliografisk kontrollert

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