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Measurement of Activity and Concentration of Paraoxonase 1 (PON-1) in Seminal Plasma and Identification of PON-2 in the Sperm of Boar Ejaculates
University of Murcia, Spain.
University of Murcia, Spain.
University of Murcia, Spain.
Linköpings universitet, Institutionen för klinisk och experimentell medicin, Avdelningen för kliniska vetenskaper. Linköpings universitet, Medicinska fakulteten.
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2015 (Engelska)Ingår i: Molecular Reproduction and Development, ISSN 1040-452X, E-ISSN 1098-2795, Vol. 82, nr 1, s. 58-65Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

This study revealed and characterised the presence of the antioxidant enzymes paraoxonase (PON) type 1 (PON-1, extracellular) and type 2 (PON-2, intracellular) in boar semen. To evaluate PON-1, an entire ejaculate from each of ten boars was collected and the seminal plasma was harvested after double centrifugation (1,500g for 10min). Seminal plasma was analysed for concentration as well as enzymatic activity of PON-1 and total cholesterol levels. Seminal-plasma PON-1 concentration ranged from 0.961 to 1.670ng/ml while its enzymatic activity ranged from 0.056 to 0.400 IU/ml, which represent individual variance. Seminal-plasma PON-1 concentration and enzymatic activity were negatively correlated (r=-0.763; Pless than0.01). The activity of seminal-plasma PON-1 negatively correlated with ejaculate volume (r=-0.726, Pless than0.05), but positively correlated with sperm concentration (r=0.654, Pless than0.05). Total seminal-plasma cholesterol concentration positively correlated with PON-1 activity (r=0.773; Pless than0.01), but negatively correlated with PON-1 concentration (r=-0.709; Pless than0.05). The presence of intracellular PON-2 was determined via immunocytochemistry in spermatozoa derived from artificial insemination. PON-2 localised to the post-acrosomal area of the sperm head and principal piece of the tail in membrane-intact spermatozoa. In summary, PON is present in boar semen, with PON-1 at low levels in seminal plasma and PON-2 within the spermatozoa. Further studies are needed to characterise the relationship between antioxidant PONs with sperm and other seminal-plasma parameters. Mol. Reprod. Dev. 82: 58-65, 2015. (c) 2014 Wiley Periodicals, Inc.

Ort, förlag, år, upplaga, sidor
Wiley-Blackwell, 2015. Vol. 82, nr 1, s. 58-65
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Medicinsk biovetenskap
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URN: urn:nbn:se:liu:diva-114595DOI: 10.1002/mrd.22444ISI: 000348656700007PubMedID: 25487823OAI: oai:DiVA.org:liu-114595DiVA, id: diva2:791430
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Funding Agencies|Ministry of Economy and Competitiveness of Spain; European Regional Development Fund [AGL2012-39903]; Swedish Research Council Formas [2011-512]; Ministry of Education, Culture and Sport of Spain [FPU12/05008]

Tillgänglig från: 2015-02-27 Skapad: 2015-02-26 Senast uppdaterad: 2017-12-04

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Rubér, MarieVicente Carrillo, AlejandroAtikuzzaman, MohammadRodriguez-Martinez, Heriberto

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Rubér, MarieVicente Carrillo, AlejandroAtikuzzaman, MohammadRodriguez-Martinez, Heriberto
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Avdelningen för kliniska vetenskaperMedicinska fakultetenAvdelningen för mikrobiologi och molekylär medicinHälsouniversitetet
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