liu.seSearch for publications in DiVA
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Proteomic and lipidomic analysis of primary mouse hepatocytes exposed to metal and metal oxide nanoparticles
Linköping University, Department of Clinical and Experimental Medicine, Division of Cell Biology. Linköping University, Faculty of Health Sciences. (Environmental proteomics, Cell Biology section)ORCID iD: 0000-0002-3894-2218
Linköping University, Department of Clinical and Experimental Medicine, Division of Cell Biology. Linköping University, Faculty of Health Sciences. (Environmental Proteomics, Cell Biology section)
Stockholm University. (Environmental Proteomics)
Stockholm University. (Environmental Proteomics)
Show others and affiliations
2015 (English)In: Journal of Integrated OMICS, ISSN 2182-0287, Vol. 5, no 1, p. 44-57Article in journal (Refereed) Published
Abstract [en]

The global analysis of the cellular lipid and protein content upon exposure to metal and metal oxide nanoparticles (NPs) can provide an overviewof the possible impact of exposure. Proteomic analysis has been applied to understand the nanoimpact however the relevance of the alterationon the lipidic proOle has been underestimated. In our study, primary mouse hepatocytes were treated with ultra-small (US) TiO2-USNPsas well as ZnO-NPs, CuO-NPs and Ag-NPs. e protein extracts were analysed by 2D-DIGE and quantiOed by imaging soPware and the selecteddi9erentially expressed proteins were identiOed by nLC-ESI-MS/MS. In parallel, lipidomic analysis of the samples was performed usingthin layer chromatography (TLC) and analyzed by imaging soPware. Our Ondings show an overall ranking of the nanoimpact at the cellularand molecular level: TiO2-USNPs<ZnO-NPs<Ag-NPs<CuO-NPs. CuO-NPs and Ag-NPs were cytotoxic while ZnO-NPs and CuO-NPs hadoxidative capacity. TiO2-USNPs did not have oxidative capacity and were not cytotoxic. e most common cellular impact of the exposurewas the down-regulation of proteins. e proteins identiOed were involved in urea cycle, lipid metabolism, electron transport chain, metabolismsignaling, cellular structure and we could also identify nuclear proteins. CuO-NPs exposure decreased phosphatidylethanolamine andphosphatidylinositol and caused down-regulation of electron transferring protein subunit beta. Ag-NPs exposure caused increased of totallipids and triacylglycerol and decrease of sphingomyelin. TiO2-USNPs also caused decrease of sphingomyelin as well as up-regulation of ATPsynthase and electron transferring protein alfa. ZnO-NPs a9ected the proteome in a concentration-independent manner with down-regulationof RNA helicase. ZnO-NPs exposure did not a9ect the cellular lipids. To our knowledge this work represents the Orst integrated proteomic andlipidomic approach to study the e9ect of NPs exposure to primary mouse hepatocytes in vitro.

Place, publisher, year, edition, pages
2015. Vol. 5, no 1, p. 44-57
Keywords [en]
nanoparticles; hepatocytes; proteomics; lipidomics; mass spectrometry; toxicity
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:liu:diva-115894DOI: 10.5584/jiomics.v5i1.184OAI: oai:DiVA.org:liu-115894DiVA, id: diva2:797186
Available from: 2015-03-23 Created: 2015-03-23 Last updated: 2015-10-28Bibliographically approved

Open Access in DiVA

fulltext(921 kB)842 downloads
File information
File name FULLTEXT01.pdfFile size 921 kBChecksum SHA-512
80240192c39fae978accef8cb6a2a76338a90297e3b37801210c151b5d9439b31c4fdc7d760ad72a491b618e08048d409a09c3f8351cd562b254137dd928f245
Type fulltextMimetype application/pdf

Other links

Publisher's full text

Authority records

Cristobal, SusanaTedesco, Sara

Search in DiVA

By author/editor
Cristobal, SusanaTedesco, Sara
By organisation
Division of Cell BiologyFaculty of Health Sciences
Biochemistry and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar
Total: 842 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 599 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf