liu.seSearch for publications in DiVA
Change search
ReferencesLink to record
Permanent link

Direct link
Microbial community adaptation influences long-chain fatty acidconversion during anaerobic codigestion of fats, oils, and grease withmunicipal sludge
Linköping University, Biogas Research Center. Civil and Environmental Engineering, University of Washington, WA, USA.
Linköping University, Biogas Research Center. Scandinavian Biogas Fuels AB, Sweden.
Science Institute, University of Washington, WA, USA.
Linköping University, Department of Thematic Studies, Tema Environmental Change. Linköping University, Biogas Research Center. Linköping University, Faculty of Arts and Sciences. Scandinavian Biogas Fuels AB, Sweden.
Show others and affiliations
2016 (English)In: Water Research, ISSN 0043-1354, E-ISSN 1879-2448, Vol. 103, 372-382 p.Article in journal (Refereed) Published
Abstract [en]

Codigesting fats, oils, and greases with municipal wastewater sludge can greatly improve biomethanerecovery at wastewater treatment facilities. Process loading rates of fats, oils, and greases have beenpreviously tested with little knowledge of the digester microbial community structure, and high transientfat loadings have led to long chain fatty acid (LCFA) accumulation and digester upsets. This studyutilized recently-developed quantitative PCR assays for syntrophic LCFA-degrading bacteria along with16S amplicon sequencing to relate changes in microbial community structure to LCFA accumulationduring transient loading increases to an anaerobic codigester receiving waste restaurant oil andmunicipal wastewater sludge. The 16S rRNA gene concentration of the syntrophic b-oxidizing genusSyntrophomonas increased to ~15% of the Bacteria community in the codigester, but stayed below 3% inthe control digester that was fed only wastewater sludge. Methanosaeta and Methanospirillum were thedominant methanogenic genera enriched in the codigester, and together comprised over 80% of theArchaea community by the end of the experimental period. Constrained ordination showed that changesin the codigester Bacteria and Archaea community structures were related to measures of digester performance.Notably, the effluent LCFA concentration in the codigester was positively correlated to thespecific loading rate of waste oil normalized to the Syntrophomonas 16S rRNA concentration. Specificloading rates of 0e1.5 1012 g VS oil/16S gene copies-day resulted in LCFA concentrations below 30 mg/g TS, whereas LCFA accumulated up to 104 mg/g TS at higher transient loading rates. Based on thecommunity-dependent loading limitations found, enhanced biomethane production from high loadingsof fats, oils and greases can be achieved by promoting a higher biomass of slow-growing syntrophicconsortia, such as with longer digester solids retention times. This work also demonstrates the potentialfor controlling the loading rate of fats, oils, and greases based on the analysis of the codigester communitystructure, such as with quantitative PCR measurements of syntrophic LCFA-degrading bacteriaabundance.

Place, publisher, year, edition, pages
2016. Vol. 103, 372-382 p.
Keyword [en]
Anaerobic digestion Fats Oils Grease (FOG) Long-chain fatty acids (LCFA) Biogas Syntrophy Methanogen
National Category
Microbiology Ecology Biochemistry and Molecular Biology
URN: urn:nbn:se:liu:diva-131778DOI: 10.1016/j.watres.2016.07.043PubMedID: 27486949OAI: diva2:1033067
Biogas Research Center, BRC-DP6
Swedish Energy Agency
Available from: 2016-10-05 Created: 2016-10-05 Last updated: 2016-10-18Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Ejlertsson, JörgenShakeri Yekta, SepehrBjörn, AnnikaSvensson, Bo H.
By organisation
Biogas Research CenterTema Environmental ChangeFaculty of Arts and Sciences
In the same journal
Water Research
MicrobiologyEcologyBiochemistry and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

ReferencesLink to record
Permanent link

Direct link