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Isolation of Cytosolic Ribosomes
Linköping University, Department of Clinical and Experimental Medicine, Division of Cell Biology. Linköping University, Faculty of Medicine and Health Sciences.
Linköping University, Department of Clinical and Experimental Medicine, Division of Cell Biology. Linköping University, Faculty of Medicine and Health Sciences.
2017 (English)In: Isolation of Plant Organelles and Structures: Methods and Protocols / [ed] Nicolas L. Taylor, A. Harvey Millar, New York: Springer, 2017, Vol. 1511, p. 241-247Chapter in book (Other academic)
Abstract [en]

This chapter describes a method of plant cytosolic ribosomes isolation typically used for further proteomic studies. Detailed description procedures including plant material disruption, various centrifugation steps, sucrose cushion centrifugation, and quality control of preparation are provided.

Place, publisher, year, edition, pages
New York: Springer, 2017. Vol. 1511, p. 241-247
Series
Methods in Molecular Biology, ISSN 1064-3745, E-ISSN 1940-6029 ; 1511
Keywords [en]
Cytosol, Ribosome, Ribosomal proteins, Sucrose cushion, Centrifugation, Ultracentrifugation
National Category
Cell Biology
Identifiers
URN: urn:nbn:se:liu:diva-132125DOI: 10.1007/978-1-4939-6533-5_19PubMedID: 27730616ISBN: 978-1-4939-6531-1 (print)ISBN: 978-1-4939-6533-5 (print)OAI: oai:DiVA.org:liu-132125DiVA, id: diva2:1037765
Available from: 2016-10-18 Created: 2016-10-18 Last updated: 2018-02-20Bibliographically approved

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