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Activity, life time and effect of hydrolytic enzymes for enhanced biogas production from sludge anaerobic digestion
Linköping University, Department of Physics, Chemistry and Biology, Chemistry. Linköping University, The Institute of Technology. Karshult Municipal Wastewater Treatment, Sweden.
Kemira Oyj, Finland.
Tekniska Verken Linkoping AB, SE-58278 Linkoping, Sweden; Swedish Meteorol and Hydrol Institute, Sweden.
Linköping University, Department of Physics, Chemistry and Biology, Chemistry. Linköping University, Faculty of Science & Engineering.
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2016 (English)In: Water Research, ISSN 0043-1354, E-ISSN 1879-2448, Vol. 103, p. 462-471Article in journal (Refereed) Published
Abstract [en]

As an alternative to energy intensive physical methods, enzymatic treatment of sludge produced at wastewater treatment plants for increased hydrolysis and biogas production was investigated. Several hydrolytic enzymes were assessed with a focus on how enzyme activity and life time was influenced by sludge environments. It could be concluded that the activity life time of added enzymes was limited (amp;lt;24 h) in both waste activated sludge and anaerobic digester sludge environments and that this was, for the majority of enzymes, due to endogenous protease activity. In biogas in situ experiments, subtilisin at a 1% mixture on basis of volatile solids, was the only enzyme providing a significantly increased biomethane production of 37%. However, even at this high concentration, subtilisin could not hydrolyze all available substrate within the life time of the enzyme. Thus, for large scale implementation, enzymes better suited to the sludge environments are needed. (C) 2016 Elsevier Ltd. All rights reserved.

Place, publisher, year, edition, pages
PERGAMON-ELSEVIER SCIENCE LTD , 2016. Vol. 103, p. 462-471
Keywords [en]
Wastewater treatment plant; Biogas; Enzyme; Protease; Subtilisin
National Category
Water Treatment
Identifiers
URN: urn:nbn:se:liu:diva-132048DOI: 10.1016/j.watres.2016.07.064ISI: 000383292100050PubMedID: 27498254OAI: oai:DiVA.org:liu-132048DiVA, id: diva2:1038470
Note

Funding Agencies|Swedish Energy Agency [P33609-1]

Available from: 2016-10-18 Created: 2016-10-17 Last updated: 2018-05-07
In thesis
1. Influencing anaerobic digestion early stage processes for increased biomethane production from different substrate components
Open this publication in new window or tab >>Influencing anaerobic digestion early stage processes for increased biomethane production from different substrate components
2018 (English)Licentiate thesis, comprehensive summary (Other academic)
Abstract [en]

Finding alternatives to petroleum-based energy sources is of interest since it could reduce the emissions of net carbon dioxide to the atmosphere by increasing the usage of renewable energy sources. To do so improvements are needed in the renewable energy production sector. Biogas production is of interest since the anaerobic digestion process can degrade many different biomolecules and is, contrary to e.g. bioethanol and biodiesel, not dependent on specific molecules. Thus, many wastes such as slaughterhouse waste, sludge from waste water treatment and lignocellulose residual material etc. can be used as substrates for biogas production. However, there are limitations in the degradation process depending on the composition of the selected substrate. To overcome these limitations such as inhibition of different microorganisms, or recalcitrant substrate, different methods can be used to increase the biogas production.

 In this study different substrates were selected and analyzed/treated for remedies of early stage rate limiting problems of the anaerobic digestion process. Different analyzes and techniques were selected depending on the limitations correlated to the main problematic component of the specific substrate.

 Improvements could be reached for the degradation of slaughterhouse waste by augmentation with the clay mineral zeolite. Addition of different enzymes to the substrate environment of different waste water treatment plant sludges resulted in limited life time of the selected enzymes. However, certain enzymes proved to be promising candidates with an effect of increased biogas production rate and yield for the time that the enzyme remained active. In an additional experiment, cellulolytic enzymes, naturally produced by a biogas producing microbial community, were induced, collected and added to a biogas experiment of ensiled forage ley, by which it was shown that these cellulases led to an increase in biogas production rate and yield. Thus, the studies demonstrate different techniques for improving the anaerobic digestion process of different types of substrates.

Place, publisher, year, edition, pages
Linköping: Linköping University Electronic Press, 2018. p. 56
Series
Linköping Studies in Science and Technology. Licentiate Thesis, ISSN 0280-7971 ; 1807
National Category
Biocatalysis and Enzyme Technology
Identifiers
urn:nbn:se:liu:diva-147721 (URN)10.3384/lic.diva-147721 (DOI)9789176852866 (ISBN)
Presentation
2018-05-31, BL21 (Nobel), Campus Valla, Linköping, 10:30 (English)
Opponent
Note

Handledare saknas

Available from: 2018-05-07 Created: 2018-05-07 Last updated: 2018-05-07Bibliographically approved

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