Surface proteomics on nanoparticles, a step to simplify the rapid prototyping of nanoparticles
2016 (English)In: Nanoscale Horizons, ISSN 2055-6756 (print), 2055-6764 (online)Article in journal (Refereed) Accepted
Engineered nanoparticles for biomedical applications requireincreasing effectiveness in targeting specific cells while preservingnon-target cell’s safety. We developed a surface proteomicsmethod for a rapid and systematic analysis of the interphasebetween the nanoparticle protein corona and the targeting cellsthat could implement the rapid prototyping of nanomedicines.Native nanoparticles entering in a protein-rich liquid mediaquickly form a macromolecular structure called protein corona.This protein structure defines the physical interaction betweennanoparticles and target cells. The surface proteins compose thefirst line of interaction between this macromolecular structureand the cell surface of a target cell. We demonstrated that SUSTU(SUrface proteomics, Safety, Targeting, Uptake) provides aqualitative and quantitative analysis from the protein coronasurface. With SUSTU, the spatial dynamics of the protein coronasurface can be studied. Data from SUSTU would ascertain thenanoparticle functionalized groups exposed at destiny that couldcircumvent preliminary in vitro experiments. Therefore thismethod could implement the analysis of nanoparticle targetingand uptake capability and could be integrated into a rapidprototyping strategy which is a major challenge in nanomaterialscience. Data are available via ProteomeXchange with identifierPXD004636.
Place, publisher, year, edition, pages
nanoparticle, protein corona, mass spectrometry, surface proteomics, targeting, rapid prototyping, nanomedicine
Biochemistry and Molecular Biology
IdentifiersURN: urn:nbn:se:liu:diva-132406DOI: 10.1039/c6nh00162aOAI: oai:DiVA.org:liu-132406DiVA: diva2:1045411
ProjectsNanoimpact; nanoparticles and rapid prototyping