liu.seSearch for publications in DiVA
Change search
ReferencesLink to record
Permanent link

Direct link
Surface proteomics on nanoparticles, a step to simplify the rapid prototyping of nanoparticles
Linköping University, Department of Clinical and Experimental Medicine, Division of Cell Biology. Linköping University, Faculty of Medicine and Health Sciences. (Cristobal Lab)
Linköping University, Department of Clinical and Experimental Medicine, Division of Cell Biology. Linköping University, Faculty of Medicine and Health Sciences. (Cristobal Lab)
Department of Biochemistry and Biophysics, Arrhenius laboratories, Stockholm University, Stockholm, Sweden. (Cristobal Lab)
Linköping University, Department of Clinical and Experimental Medicine, Division of Cell Biology. Linköping University, Faculty of Medicine and Health Sciences. IKERBASQUE, Basque Foundation for Science, Department of Physiology, Faculty of Medicine and Dentristy, University of the Basque Country, Leioa, Spain. (Cristobal Lab)ORCID iD: 0000-0002-3894-2218
2016 (English)In: Nanoscale Horizons, ISSN 2055-6756 (print), 2055-6764 (online)Article in journal (Refereed) Accepted
Abstract [en]

Engineered nanoparticles for biomedical applications requireincreasing effectiveness in targeting specific cells while preservingnon-target cell’s safety. We developed a surface proteomicsmethod for a rapid and systematic analysis of the interphasebetween the nanoparticle protein corona and the targeting cellsthat could implement the rapid prototyping of nanomedicines.Native nanoparticles entering in a protein-rich liquid mediaquickly form a macromolecular structure called protein corona.This protein structure defines the physical interaction betweennanoparticles and target cells. The surface proteins compose thefirst line of interaction between this macromolecular structureand the cell surface of a target cell. We demonstrated that SUSTU(SUrface proteomics, Safety, Targeting, Uptake) provides aqualitative and quantitative analysis from the protein coronasurface. With SUSTU, the spatial dynamics of the protein coronasurface can be studied. Data from SUSTU would ascertain thenanoparticle functionalized groups exposed at destiny that couldcircumvent preliminary in vitro experiments. Therefore thismethod could implement the analysis of nanoparticle targetingand uptake capability and could be integrated into a rapidprototyping strategy which is a major challenge in nanomaterialscience. Data are available via ProteomeXchange with identifierPXD004636.

Place, publisher, year, edition, pages
2016.
Keyword [en]
nanoparticle, protein corona, mass spectrometry, surface proteomics, targeting, rapid prototyping, nanomedicine
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:liu:diva-132406DOI: 10.1039/c6nh00162aOAI: oai:DiVA.org:liu-132406DiVA: diva2:1045411
Projects
Nanoimpact; nanoparticles and rapid prototyping
Available from: 2016-11-09 Created: 2016-11-09 Last updated: 2016-11-14Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Kuruvilla, JacobFarinha, Ana PaulaBayat, NargesCristobal, Susana
By organisation
Division of Cell BiologyFaculty of Medicine and Health Sciences
Biochemistry and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar

Altmetric score

Total: 29 hits
ReferencesLink to record
Permanent link

Direct link