Depression-like behavior in rat: Involvement of galanin receptor subtype 1 in the ventral periaqueductal gray
2016 (English)In: Proceedings of the National Academy of Sciences of the United States of America, ISSN 0027-8424, E-ISSN 1091-6490, Vol. 113, no 32, E4726-E4735 p.Article in journal (Refereed) Published
The neuropeptide galanin coexists in rat brain with serotonin in the dorsal raphe nucleus and with noradrenaline in the locus coeruleus (LC), and it has been suggested to be involved in depression. We studied rats exposed to chronic mild stress (CMS), a rodent model of depression. As expected, these rats showed several endophenotypes relevant to depression-like behavior compared with controls. All these endophenotypes were normalized after administration of a selective serotonin reuptake inhibitor. The transcripts for galanin and two of its receptors, galanin receptor 1 (GALR1) and GALR2, were analyzed with quantitative real-time PCR using laser capture microdissection in the following brain regions: the hippocampal formation, LC, and ventral periaqueductal gray (vPAG). Only Galr1 mRNA levels were significantly increased, and only in the latter region. After knocking down Galr1 in the vPAG with an siRNA technique, all parameters of the depressive behavioral phenotype were similar to controls. Thus, the depression-like behavior in rats exposed to CMS is likely related to an elevated expression of Galr1 in the vPAG, suggesting that a GALR1 antagonist could have antidepressant effects.
Place, publisher, year, edition, pages
NATL ACAD SCIENCES , 2016. Vol. 113, no 32, E4726-E4735 p.
dorsal raphe; neuropeptide; siRNA; stress; transmitter coexistence
IdentifiersURN: urn:nbn:se:liu:diva-132490DOI: 10.1073/pnas.1609198113ISI: 000381293300021PubMedID: 27457954OAI: oai:DiVA.org:liu-132490DiVA: diva2:1046257
Funding Agencies|National Natural Sciences Foundation of China [30870815, 31171032]; Beijing Natural Science Foundation ; National Basic Research Program of China 973 Program [2010CB912003]; Beijing Talent Project [PHR20100510]; Swedish Research Council [4X-2887]; Seed Grant of the International Alliance of Translational Neuroscience [PXM2014_014226_000006]; Beijing Brain Project [Z161100000216142]; KI2016-11-132016-11-122016-11-13