Identification of Stage-Specific Surface Markers in Early B Cell Development Provides Novel Tools for Identification of Progenitor Populations
2016 (English)In: Journal of Immunology, ISSN 0022-1767, E-ISSN 1550-6606, Vol. 197, no 5, 1937-1944 p.Article in journal (Refereed) Published
Whereas the characterization of B lymphoid progenitors has been facilitated by the identification of lineage- nd stage-specific surface markers, the continued identification of differentially expressed proteins increases our capacity to explore normal and malignant B cell development. To identify novel surface markers with stage-specific expression patterns, we explored the reactivity of CD19(+) B cell progenitor cells to Abs targeted to 176 surface proteins. Markers with stage-specific expression were identified using a transgenic reporter gene system subdividing the B cell progenitors into four surface IgM(-) stages. This approach affirmed the utility of known stage-specific markers, as well as identifying additional proteins that selectively marked defined stages of B cell development. Among the stage-specific markers were the cell adhesion proteins CD49E, CD11A, and CD54 that are highly expressed selectively on the most immature progenitors. This work identifies a set of novel stage-specific surface markers that can be used as a complement to the classical staining protocols to explore B lymphocyte development.
Place, publisher, year, edition, pages
AMER ASSOC IMMUNOLOGISTS , 2016. Vol. 197, no 5, 1937-1944 p.
Cell and Molecular Biology
IdentifiersURN: urn:nbn:se:liu:diva-132484DOI: 10.4049/jimmunol.1600297ISI: 000385002300041PubMedID: 27456481OAI: oai:DiVA.org:liu-132484DiVA: diva2:1046262