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Quantitative analysis of immunogold labeling indicates low levels and non-vesicular localization of L-aspartate in rat primary afferent terminals.
Department of Physiological Sciences, Lund University, Lund, Sweden.ORCID iD: 0000-0003-3584-7829
Department of Physiological Sciences, Lund University, Lund, Sweden.
Department of Anatomy, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway.
Department of Physiological Sciences, Lund University, Lund, Sweden.
2001 (English)In: Journal of Comparative Neurology, ISSN 0021-9967, E-ISSN 1096-9861, Vol. 430, no 2, 147-159 p.Article in journal (Refereed) Published
Abstract [en]

The role of L-aspartate as an excitatory neurotransmitter in primary afferent synapses in the spinal cord dorsal horn is disputed. To further investigate this issue, we examined the presence of aspartate-like immunoreactivity in primary afferent nerve terminals and other tissue components of the dorsal horn. We also examined the relationship between aspartate and glutamate immunogold labeling density and the density of synaptic vesicles in primary afferent terminals and presumed inhibitory terminals forming symmetric synapses. Weak aspartate immunosignals, similar to or lower than those displayed by presumed inhibitory terminals, were detected in both C-fiber primary afferent terminals in lamina II (dense sinusoid axon terminals, identified by morphological criteria) and in A-fiber primary afferent terminals in laminae III-IV (identified with anterograde transport of choleragenoid-horseradish peroxidase conjugate). The aspartate immunogold signal in primary afferent terminals was only about one-fourth of that in deep dorsal horn neuronal cell bodies. Further, whereas significant positive correlations were evident between synaptic vesicle density and glutamate immunogold labeling density in both A- and C-fiber primary afferent terminals, none of the examined terminal populations displayed a significant correlation between synaptic vesicle density and aspartate immunogold labeling density. Thus, our results indicate relatively low levels and a non-vesicular localization of aspartate in primary afferent terminals. It is therefore suggested that aspartate, rather than being a primary afferent neurotransmitter, serves a role in the intermediary metabolism in primary afferent terminals.

Place, publisher, year, edition, pages
John Wiley & Sons, 2001. Vol. 430, no 2, 147-159 p.
National Category
Neurosciences
Identifiers
URN: urn:nbn:se:liu:diva-133061DOI: 10.1002/1096-9861(20010205)430:2<147::AID-CNE1021>3.0.CO;2-5ISI: 000166232700001PubMedID: 11135252Scopus ID: 2-s2.0-0035809121OAI: oai:DiVA.org:liu-133061DiVA: diva2:1053218
Available from: 2016-12-08 Created: 2016-12-08 Last updated: 2016-12-13Bibliographically approved

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