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Functional and anatomical identification of a vesicular transporter mediating neuronal ATP release.
Department of Anatomy and Centre for Molecular Biology and Neuroscience, University of Oslo, Oslo, Norway.ORCID iD: 0000-0003-3584-7829
Department of Membrane Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
Department of Anatomy and Centre for Molecular Biology and Neuroscience, University of Oslo, Oslo, Norway.
Department of Membrane Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
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2012 (English)In: Cerebral Cortex, ISSN 1047-3211, E-ISSN 1460-2199, Vol. 22, no 5, 1203-1214 p.Article in journal (Refereed) Published
Abstract [en]

ATP is known to be coreleased with glutamate at certain central synapses. However, the nature of its release is controversial. Here, we demonstrate that ATP release from cultured rat hippocampal neurons is sensitive to RNAi-mediated knockdown of the recently identified vesicular nucleotide transporter (VNUT or SLC17A9). In the intact brain, light microscopy showed particularly strong VNUT immunoreactivity in the cerebellar cortex, the olfactory bulb, and the hippocampus. Using immunoelectron microscopy, we found VNUT immunoreactivity colocalized with synaptic vesicles in excitatory and inhibitory terminals in the hippocampal formation. Moreover, VNUT immunolabeling, unlike that of the vesicular glutamate transporter VGLUT1, was enriched in preterminal axons and present in postsynaptic dendritic spines. Immunoisolation of synaptic vesicles indicated presence of VNUT in a subset of VGLUT1-containing vesicles. Thus, we conclude that VNUT mediates transport of ATP into synaptic vesicles of hippocampal neurons, thereby conferring a purinergic phenotype to these cells.

Place, publisher, year, edition, pages
2012. Vol. 22, no 5, 1203-1214 p.
Keyword [en]
exocytosis, neurotransmitter, purinergic, ultrastructure, uptake
National Category
Neurosciences
Identifiers
URN: urn:nbn:se:liu:diva-133049DOI: 10.1093/cercor/bhr203ISI: 000303161400022PubMedID: 21810784Scopus ID: 2-s2.0-84860144966OAI: oai:DiVA.org:liu-133049DiVA: diva2:1054579
Available from: 2016-12-08 Created: 2016-12-08 Last updated: 2016-12-13Bibliographically approved

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