liu.seSearch for publications in DiVA
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Glutathione Peroxidase 5 Is Expressed by the Entire Pig Male Genital Tract and Once in the Seminal Plasma Contributes to Sperm Survival and In Vivo Fertility
University of Murcia, Spain.
University of Murcia, Spain.
University of Murcia, Spain.
Linköping University, Department of Clinical and Experimental Medicine, Division of Clinical Sciences. Linköping University, Faculty of Medicine and Health Sciences.ORCID iD: 0000-0002-0256-1958
Show others and affiliations
2016 (English)In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 11, no 9, article id e0162958Article in journal (Refereed) Published
Abstract [en]

Glutathione peroxidase-5 (GPX5) is an H2O2-scavenging enzyme identified in boar seminal plasma (SP). This study attempted to clarify its origin and role on sperm survival and fertility after artificial insemination (AI). GPX5 was expressed (Western blot and immunocytochemistry using a rabbit primary polyclonal antibody) in testes, epididymis and accessory sex glands (6 boars). SP-GPX5 concentration differed among boars (11 boars, P amp;lt; 0.001), among ejaculates within boar (44 ejaculates, P amp;lt; 0.001) and among portions within ejaculate (15 ejaculates). The first 10 mL of the spermrich fraction (SRF, sperm-peak portion) had a significantly lower concentration (8.87 +/- 0.78 ng/mL) than the rest of the SRF and the post-SRF (11.66 +/- 0.79 and 12.37 +/- 0.79 ng/mL, respectively, P amp;lt; 0.005). Spermmotility of liquid-stored semen AI-doses (n = 44, at 15-17 degrees C during 72h) declined faster in AI-doses with low concentrations of SP-GPX5 compared to those with high-levels. Boars (n = 11) with high SP-GPX5 showed higher farrowing rates and litter sizes than those with low SP-GPX5 (a total of 5,275 inseminated sows). In sum, GPX5 is widely expressed in the boar genital tract and its variable presence in SP shows a positive relationship with sperm quality and fertility outcomes of liquid-stored semen AI-doses.

Place, publisher, year, edition, pages
PUBLIC LIBRARY SCIENCE , 2016. Vol. 11, no 9, article id e0162958
National Category
Clinical Laboratory Medicine
Identifiers
URN: urn:nbn:se:liu:diva-133125DOI: 10.1371/journal.pone.0162958ISI: 000383680600068PubMedID: 27627110OAI: oai:DiVA.org:liu-133125DiVA, id: diva2:1055247
Note

Funding Agencies|Ministerio de Economia y Competitividad Madrid (Spain) [AGL2012-39903, AGL2015-68738-R]; Seneca Foundation Murcia (Spain) [19892/GERM/15]; Swedish Research Council (VR) [521-2011-6353]; Swedish Research Council Formas [221-2011-512]; Research Council in Southeast Sweden (FORSS), Sweden [378091/312971]; Ministerio de Educacion, Cultura y Deporte, Spain; Fundacion Seneca, Murcia, Spain

Available from: 2016-12-12 Created: 2016-12-09 Last updated: 2017-11-29

Open Access in DiVA

fulltext(2802 kB)39 downloads
File information
File name FULLTEXT01.pdfFile size 2802 kBChecksum SHA-512
d21190362f72b0b1dcab1fac1ad9555bbd6e2cd767fdf7a7f9e91f58ddc9cf4944f01f17f9368708e48696bb9835a26db49db774affb4ef57027e06f8e66f6d8
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Vicente Carrillo, AlejandroRodriguez-Martinez, Heriberto
By organisation
Division of Clinical SciencesFaculty of Medicine and Health Sciences
In the same journal
PLoS ONE
Clinical Laboratory Medicine

Search outside of DiVA

GoogleGoogle Scholar
Total: 39 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 148 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf