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Capturing Excited States in the Fast-Intermediate Exchange Limit in Biological Systems Using (HNMR)-H-1 Spectroscopy
Karolinska Institute, Sweden.
Karolinska Institute, Sweden.
Linköping University, Department of Physics, Chemistry and Biology, Chemistry. Linköping University, Faculty of Science & Engineering.
Karolinska Institute, Sweden.
2016 (English)In: ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, ISSN 1433-7851, Vol. 55, no 51, 15869-15872 p.Article in journal (Refereed) Published
Abstract [en]

Changes in molecular structure are essential for the function of biomolecules. Characterization of these structural fluctuations can illuminate alternative states and help in correlating structure to function. NMR relaxation dispersion (RD) is currently the only method for detecting these alternative, high-energy states. In this study, we present a versatile H-1 R-1 RD experiment that not only extends the exchange timescales at least three times beyond the rate limits of C-13/N-15 R-1 and ten times for CPMG experiments, but also makes use of easily accessible probes, thus allowing a general description of biologically important excited states. This technique can be used to extract chemical shifts for the structural characterization of excited states and to elucidate complex excited states.

Place, publisher, year, edition, pages
WILEY-V C H VERLAG GMBH , 2016. Vol. 55, no 51, 15869-15872 p.
Keyword [en]
biomolecular dynamics; excited states; H-1 NMR spectroscopy; nucleic acids; relaxation dispersion
National Category
Theoretical Chemistry
Identifiers
URN: urn:nbn:se:liu:diva-134082DOI: 10.1002/anie.201609102ISI: 000390599400029PubMedID: 27860024OAI: oai:DiVA.org:liu-134082DiVA: diva2:1068892
Note

Funding Agencies|Swedish Research Council [2012-5136]; Tryggers foundation [CTS14-383, CTS15-388]; Karolinska Institutet; Swedish Foundation for Strategic Research [ICA 14-0023]; Ragnar Soderberg foundation [M91/14]; Karolinska Institute; MBB Department

Available from: 2017-01-26 Created: 2017-01-22 Last updated: 2017-02-20

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  • apa
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More styles
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  • de-DE
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