Erythrocyte fatty acid composition does not influence levels of free, bioavailable, and total 25-hydroxy vitamin DShow others and affiliations
2017 (English)In: SCANDINAVIAN JOURNAL OF CLINICAL and LABORATORY INVESTIGATION, ISSN 0036-5513, Vol. 77, no 1, p. 45-52Article in journal (Refereed) Published
Abstract [en]
In vitro, mono- and polyunsaturated fatty acids (FAs) may decrease the binding affinity of vitamin D metabolites for vitamin D-binding protein, which in turn may influence their bioavailability. FAs incorporated as phospholipids in erythrocyte (ery-) cell membranes reflect dietary intake. The purpose of this study was to investigate ery-FA composition in relation to markers for vitamin D. In healthy females (age 22.6 +/- 2.0 years) total 25(OH)D was measured by LC-MS/MS (n=78), free 25(OH)D with ELISA (n=64 of 78), and bioavailable 25(OH)D was calculated. Analysis of ery-FA composition was by gas chromatography (n=56 of 78). A strong correlation between total 25(OH)D and free 25(OH)D was seen (r=.66, pamp;lt;.001), and between total-25(OH)D and bioavailable 25(OH)D (r=.68, pamp;lt;.001). No correlations between 25(OH)D fractions and specific fatty acids were found, and in particular, no associations with mono- and poly-unsaturated FA compositions. All 25(OH)D fractions were correlated with leptin (total 25(OH)D (r=-.33, pamp;lt;.003); bioavailable 25(OH)D (r=-.47, pamp;lt;.001); free 25(OH)D (r=-.44, pamp;lt;.001). Associations were found between PTH and total 25(OH)D (r=-.35, p=.002) and weaker between bioavailable 25(OH)D (r=-.35, p=.040) and free 25(OH)D (r=-.28, p=.079). All fractions of 25(OH)D appear to correlate in a similar way to PTH, BMI and body fat (leptin). No association was found between ery-FA composition and free/bioavailable 25(OH)D. It is unlikely that FAs are a strong uncoupling factor of DBP-bound 25(OH)D.
Place, publisher, year, edition, pages
TAYLOR & FRANCIS LTD , 2017. Vol. 77, no 1, p. 45-52
Keywords [en]
Erythrocyte membrane; fatty acids; leptin; lipids; parathyroid hormone; Vitamin D
National Category
Nutrition and Dietetics
Identifiers
URN: urn:nbn:se:liu:diva-134210DOI: 10.1080/00365513.2016.1258724ISI: 000390847200009PubMedID: 27905210OAI: oai:DiVA.org:liu-134210DiVA, id: diva2:1069829
Note
Funding Agencies|Medical Council of Southeast Sweden (FORSS)
2017-01-302017-01-292017-01-30