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Ethylene glycol, but not DMSO, could replace glycerol inclusion in soybean lecithin-based extenders in ram sperm cryopreservation.
Department of Animal Science, College of Agriculture, University of Tabriz, Tabriz, Iran.
Department of Animal Science, College of Agriculture, University of Tabriz, Tabriz, Iran.
Department of Animal Science, College of Agriculture, University of Tabriz, Tabriz, Iran.
Department of Animal Science, College of Agriculture, University of Tabriz, Tabriz, Iran.
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2017 (English)In: Animal Reproduction Science, ISSN 0378-4320, E-ISSN 1873-2232, Vol. 177, 35-41 p.Article in journal (Refereed) Published
Abstract [en]

The aim of this study was to evaluate the effects of glycerol, ethylene glycol or DMSO in a soybean lecithin extender for freezing ram semen. In this study, 20 ejaculates were collected from four Ghezel rams and diluted with soybean lecithin extender with glycerol (7%), ethylene glycol (3%, 5% and 7%) or DMSO (3%, 5% and 7%). Sperm motility (CASA), membrane integrity (HOS test), viability, total abnormality, mitochondrial activity (Rhodamine 123) and apoptotic features (Annexin V/Propidium iodide) were assessed after thawing. There was no significant difference between glycerol and ethylene glycol at different concentrations (3% and 5%) regarding sperm total and progressive motility, viability, and membrane integrity. The least percentages of mitochondrial functionality were observed in samples frozen with all different DMSO concentrations tested (P<0.05). Moreover, the percentage of post-thawed dead sperm was the greatest for all the DMSO concentrations compared with other groups (P<0.05). Thus, DMSO had an adverse effect on the post thaw ram sperm parameters. In contrast, ethylene glycol could be a desirable substitute of glycerol in the freezing extender, in view of similar results obtained in post-thaw quality of ram semen cryopreserved in a soybean lecithin extender. We propose that glycerol in a soybean lecithin based extender could be replaced by ethylene glycol at 3% or 5% concentrations.

Place, publisher, year, edition, pages
Elsevier, 2017. Vol. 177, 35-41 p.
National Category
Biological Sciences
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URN: urn:nbn:se:liu:diva-134397DOI: 10.1016/j.anireprosci.2016.12.004ISI: 000393267800004PubMedID: 28011116OAI: oai:DiVA.org:liu-134397DiVA: diva2:1076656
Available from: 2017-02-23 Created: 2017-02-23 Last updated: 2017-11-29

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Alvarez-Rodriguez, Manuel
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