Evaluation of tubulin β-3 as a novel senescence-associated gene in melanocytic malignant transformation.
2017 (English)In: Pigment Cell & Melanoma Research, ISSN 1755-1471, E-ISSN 1755-148X, Vol. 30, no 2, 243-254 p.Article in journal (Refereed) Published
Malignant melanoma might develop from melanocytic nevi in which the growth-arrested state has been broken. We analyzed the gene expression of young and senescent human melanocytes in culture and compared the gene expression data with a dataset from nevi and melanomas. A concordant altered gene expression was identified in 84 genes when comparing the growth-arrested samples with proliferating samples. TUBB3, which encodes the microtubule protein tubulin β-3, showed a decreased expression in senescent melanocytes and nevi and was selected for further studies. Depletion of tubulin β-3 caused accumulation of cells in the G2/M phase and decreased proliferation and migration. Immunohistochemical assessment of tubulin β-3 in benign lesions revealed strong staining in the superficial part of the intradermal components, which faded with depth. In contrast, primary melanomas exhibited staining without gradient in a disordered pattern and strong staining of the invasive front. Our results describe an approach to find clinically useful diagnostic biomarkers to more precisely identify cutaneous malignant melanoma and present tubulin β-3 as a candidate marker. This article is protected by copyright. All rights reserved.
Place, publisher, year, edition, pages
Blackwell Munksgaard, 2017. Vol. 30, no 2, 243-254 p.
senescence, nevus, melanoma, microarray, tubulin β-3
Cell and Molecular Biology
IdentifiersURN: urn:nbn:se:liu:diva-134870DOI: 10.1111/pcmr.12572PubMedID: 28024114ScopusID: 2-s2.0-85014598335OAI: oai:DiVA.org:liu-134870DiVA: diva2:1077544