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Evaluation of tubulin β-3 as a novel senescence-associated gene in melanocytic malignant transformation.
Linköping University, Department of Clinical and Experimental Medicine, Division of Cell Biology. Linköping University, Faculty of Medicine and Health Sciences. Region Östergötland, Heart and Medicine Center, Department of Dermatology and Venerology.
Linköping University, Department of Clinical and Experimental Medicine, Division of Cell Biology. Linköping University, Faculty of Medicine and Health Sciences.
Linköping University, Department of Clinical and Experimental Medicine, Division of Cell Biology. Linköping University, Faculty of Medicine and Health Sciences. Region Östergötland, Center for Diagnostics, Department of Clinical Pathology and Clinical Genetics.
Linköping University, Department of Clinical and Experimental Medicine, Division of Cell Biology. Linköping University, Faculty of Medicine and Health Sciences. Region Östergötland, Heart and Medicine Center, Department of Dermatology and Venerology.
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2017 (English)In: Pigment Cell & Melanoma Research, ISSN 1755-1471, E-ISSN 1755-148X, Vol. 30, no 2, 243-254 p.Article in journal (Refereed) Published
Abstract [en]

Malignant melanoma might develop from melanocytic nevi in which the growth-arrested state has been broken. We analyzed the gene expression of young and senescent human melanocytes in culture and compared the gene expression data with a dataset from nevi and melanomas. A concordant altered gene expression was identified in 84 genes when comparing the growth-arrested samples with proliferating samples. TUBB3, which encodes the microtubule protein tubulin β-3, showed a decreased expression in senescent melanocytes and nevi and was selected for further studies. Depletion of tubulin β-3 caused accumulation of cells in the G2/M phase and decreased proliferation and migration. Immunohistochemical assessment of tubulin β-3 in benign lesions revealed strong staining in the superficial part of the intradermal components, which faded with depth. In contrast, primary melanomas exhibited staining without gradient in a disordered pattern and strong staining of the invasive front. Our results describe an approach to find clinically useful diagnostic biomarkers to more precisely identify cutaneous malignant melanoma and present tubulin β-3 as a candidate marker. This article is protected by copyright. All rights reserved.

Place, publisher, year, edition, pages
Blackwell Munksgaard, 2017. Vol. 30, no 2, 243-254 p.
Keyword [en]
senescence, nevus, melanoma, microarray, tubulin β-3
National Category
Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:liu:diva-134870DOI: 10.1111/pcmr.12572ISI: 000397412300010PubMedID: 28024114Scopus ID: 2-s2.0-85014598335OAI: oai:DiVA.org:liu-134870DiVA: diva2:1077544
Note

Funding agencies: Swedish Research Council; Welander-Finsen Foundation; Ostgotaregionens Cancer Foundation; Swedish Cancer Society; County Council of Ostergotland; Olle Engkvist Foundation

Available from: 2017-02-28 Created: 2017-02-28 Last updated: 2017-04-20Bibliographically approved

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The full text will be freely available from 2018-03-07 14:18
Available from 2018-03-07 14:18

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Orfanidis, KyriakosWäster, PetraLundmark, KatarzynaRosdahl, IngerÖllinger, Karin
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