Transforming Growth Factor Beta Gene Signatures are Spatially Enriched in Keloid Tissue Biopsies and Ex vivo-Cultured Keloid Fibroblasts
2017 (English)In: Acta Dermato-Venereologica, ISSN 0001-5555, E-ISSN 1651-2057, Vol. 97, no 1, 10-16 p.Article in journal (Refereed) Published
The keloid lesion is recognised as a spatially heterogeneous mass both in cellular and acellular composition and biological activity. Here, we have utilised a bioinformatic approach to determine whether this spatial heterogeneity is also evident at the molecular level and to identify key upstream regulators of signalling pathways enriched in the lesion in a spatially-restricted manner. Differentially expressed genes (20% change, p amp;lt; 0.05) obtained from microarray datasets derived from whole keloid biopsies and ex vivo-cultured keloid fibroblasts, both from distinct regions of the keloid lesion (leading edge, centre, and top) have been analysed to show that the TGF beta family plays a significant but spatially dependent role in regulation of keloid gene expression. Furthermore, we have identified additional upstream signalling molecules involved in driving keloid biology and provide information on therapeutic targets whose modulation might be expected to lead to significant therapeutic efficacy.
Place, publisher, year, edition, pages
ACTA DERMATO-VENEREOLOGICA , 2017. Vol. 97, no 1, 10-16 p.
keloid disease; gene expression; microarray; TGF beta 1; upstream regulator
Cell and Molecular Biology
IdentifiersURN: urn:nbn:se:liu:diva-136356DOI: 10.2340/00015555-2462ISI: 000393894700004PubMedID: 27175945OAI: oai:DiVA.org:liu-136356DiVA: diva2:1087866