Fucoidan-Mimetic Glycopolymers as Tools for Studying Molecular and Cellular Responses in Human Blood Platelets
2017 (English)In: Macromolecular Bioscience, ISSN 1616-5187, E-ISSN 1616-5195, Vol. 17, no 2, UNSP 1600257Article in journal (Refereed) Published
The marine sulfated polysaccharide fucoidan displays superior ability to induce platelet aggregation compared to other sulfated polysaccharides. As such, it is an attractive tool for studying molecular and cellular responses in activated platelets. The heterogeneous structure, however, poses a problem in such applications. This study describes the synthesis of sulfated alpha-L-fucoside-pendant poly(methacryl amides) with homogeneous structures. By using both thiol-mediated chain transfer and reversible addition-fragmentation chain transfer polymerization techniques, glycopolymers with different chain lengths are obtained. These glycopolymers show platelet aggregation response and surface changes similar to those of fucoidan, and cause platelet activation through intracellular signaling as shown by extensive protein tyrosine phosphorylation. As the platelet activating properties of the glycopolymers strongly mimic those of fucoidan, this study concludes these fucoidan-mimetic glycopolymers are unique tools for studying molecular and cellular responses in human blood platelets.
Place, publisher, year, edition, pages
WILEY-V C H VERLAG GMBH , 2017. Vol. 17, no 2, UNSP 1600257
biological applications of polymers; biomimetic; radical polymerization; reversible addition fragmentation chain transfer; structure-property relations
IdentifiersURN: urn:nbn:se:liu:diva-136337DOI: 10.1002/mabi.201600257ISI: 000394592600012PubMedID: 27616165OAI: oai:DiVA.org:liu-136337DiVA: diva2:1087905
Funding Agencies|AFA Insurance, VR Treatments of the Future grant2017-04-102017-04-102017-04-10