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Tissue Harvesting Site and Culture Medium Affect Attachment, Growth, and Phenotype of Ex Vivo Expanded Oral Mucosal Epithelial Cells
Harvard Medical Sch, MA USA; University of Oslo, Norway; Oslo University Hospital, Norway.
Oslo University Hospital, Norway.
Harvard Medical Sch, MA USA; University of Coll Southeast Norway, Norway; Innlandet Hospital Trust, Norway.
Harvard Medical Sch, MA USA; Oslo University Hospital, Norway; University of Oslo, Norway.
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2017 (English)In: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 7, 674Article in journal (Refereed) Published
Abstract [en]

Transplantation of cultured oral mucosal epithelial cells (OMECs) is a promising treatment strategy for limbal stem cell deficiency. In order to improve the culture method, we investigated the effects of four culture media and tissue harvesting sites on explant attachment, growth, and phenotype of OMECs cultured from Sprague-Dawley rats. Neither choice of media or harvesting site impacted the ability of the explants to attach to the culture well. Dulbeccos modified Eagles medium/Hams F12 (DMEM) and Roswell Park Memorial Institute 1640 medium (RPMI) supported the largest cellular outgrowth. Fold outgrowth was superior from LL explants compared to explants from the buccal mucosa (BM), HP, and transition zone of the lower lip (TZ) after six-day culture. Putative stem cell markers were detected in cultures grown in DMEM and RPMI. In DMEM, cells from TZ showed higher colony-forming efficiency than LL, BM, and HP. In contrast to RPMI, DMEM both expressed the putative stem cell marker Bmi-1 and yielded cell colonies. Our data suggest that OMECs from LL and TZ cultured in DMEM give rise to undifferentiated cells with high growth capacity, and hence are the most promising for treatment of limbal stem cell deficiency.

Place, publisher, year, edition, pages
NATURE PUBLISHING GROUP , 2017. Vol. 7, 674
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Cell and Molecular Biology
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URN: urn:nbn:se:liu:diva-136861DOI: 10.1038/s41598-017-00417-zISI: 000398545400018PubMedID: 28386069OAI: oai:DiVA.org:liu-136861DiVA: diva2:1092111
Available from: 2017-04-30 Created: 2017-04-30 Last updated: 2017-05-22

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