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An integrated dual functional recognition/amplification bio-label for the one-step impedimetric detection of Micro-RNA-21
Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering. University of Sousse, Tunisia.
Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering.ORCID iD: 0000-0003-3274-6029
Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering. Iranian National Institute Oceanog and Atmospher Science, Iran.
Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering.ORCID iD: 0000-0002-1815-9699
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2017 (English)In: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 92, 154-161 p.Article in journal (Refereed) Published
Abstract [en]

Alteration in expression of miRNAs has been correlated with different cancer types, tumour stage and response to treatments. In this context, a structurally responsive oligonucleotide-based electrochemical impedimetric biosensor has been developed for the simple and sensitive detection of miRNA-21. A highly specific biotinylated DNA/LNA molecular beacon (MB) probe was conjugated with gold nanoparticles (AuNPs) to create an integrated, dual function bio-label (biotin-MB-AuNPs) for both biorecognition and signal generation. In the presence of target miRNA-21, hybridisation takes place resulting in the "activation" of the biotin-MB; this event makes the biotin group, which was previously "protected" by the steric hindrance of the MB stem-loop structure, accessible. The activated biotin-MB-AuNPs/miRNA complexes become available for capture, via supramolecular interaction, onto a nentravidin-modified electrode for electrochemical transduction. The binding event results in a decrease of the charge transfer resistance at the working electrode/electrolyte interface. The biosensor responded linearly in the range 1-1000 pM of miRNA-21, with a limit of detection of 0.3 pM, good reproducibility (Relative Standard deviation (RSD) =3.3%) and high selectivity over other miRNAs (i.e. miRNA221 and miRNA-205) sequences. Detection of miRNA-21 in spiked serum samples at clinically relevant levels (low pM range) was also demonstrated, thus illustrating the potential of the biosensor for point-of-care clinical applications. The proposed biosensor design, based on the combination of a neutravidin transducing surface and the dual-function biotin-MB-AuNPs bio-label, provides a simple and robust approach for detection of short-length nucleic acid targets, such as miRNAs.

Place, publisher, year, edition, pages
ELSEVIER ADVANCED TECHNOLOGY , 2017. Vol. 92, 154-161 p.
Keyword [en]
MicroRNA-21; Gold nanoparticles; Molecular beacon; Electrochemical impedance spectroscopy
National Category
Analytical Chemistry
Identifiers
URN: urn:nbn:se:liu:diva-136850DOI: 10.1016/j.bios.2017.02.014ISI: 000396946600023PubMedID: 28213328OAI: oai:DiVA.org:liu-136850DiVA: diva2:1092119
Note

Funding Agencies|European Communities Seventh Framework Program [PIRSES-GA-2012-318053]

Available from: 2017-04-30 Created: 2017-04-30 Last updated: 2017-05-22

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The full text will be freely available from 2019-02-10 10:23
Available from 2019-02-10 10:23

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CiteExportLink to record
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