liu.seSearch for publications in DiVA
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
The impact of DIDS-induced inhibition of voltage-dependent anion channels (VDAC) on cellular response of lymphoblastoid cells to ionizing radiation.
Silesian University of Technology.
Centre for Molecular Medicine, Norway .
S.P.Kapitsa Technological Research Institute.
Collegium Medicum in Bydgoszcz, Poland.
Show others and affiliations
2017 (English)In: Medicinal chemistry, ISSN 1573-4064, Vol. 13, no 5, p. 477-483Article in journal (Refereed) Published
Abstract [en]

Background: The voltage-dependent ion channels (VDAC) play an essential role in the cross talk between mitochondria and the rest of the cell. Their implication in cell life and cell death has been studied extensively in recent years. In this work we studied the impact of mitochondrial membrane voltage-dependent anion channels (VDACs) on cell survival and response to X-ionizing radiation (IR) of human lymphoblastoid K562 cells. Methods: The inhibition of VDACs was achieved by 4,4′-diisothiocyanostilbene-2,2′-disulfonic acid (DIDS) inhibitor and in vitro experiments including clonogenity assay, UV-visible spectrophotometry, comet assay and FACS analysis were implemented. Results: Inhibition of VDAC led to augmentation of IR-induced apoptosis and ROS production. Additionally, DIDS affected repair of IR-induced DNA strand breaks and was in line with both induction of apoptosis and caspase activity. The IR-induced NO production was potently reduced by inhibition of VDAC. Conclusion: Our results suggest that VDAC control cellular response to ionizing radiation through modulation of the ROS- and NO-dependent signaling pathways. Inhibition of VDAC with DIDS induced apoptosis in irradiated K562 lymphoblastoid cells points at DIDS, as a promising agent to enhance the effectiveness of radiotherapy.

Place, publisher, year, edition, pages
Bentham Science Publishers Ltd. , 2017. Vol. 13, no 5, p. 477-483
Keywords [en]
4, 4′-diisothiocyanostilbene-2, 2′-disulfonic acid (DIDS); voltage-dependent anion channel (VDAC); reactive oxygen species (ROS), ionizing radiation, cell death, DNA strand breaks.
National Category
Health Sciences
Identifiers
URN: urn:nbn:se:liu:diva-137275DOI: 10.2174/1573406413666170421102353ISI: 000405545000008PubMedID: 28427245OAI: oai:DiVA.org:liu-137275DiVA, id: diva2:1094430
Note

Funding agencies: National Science Center [DEC-2012/07/B/NZ1/00008, NCN 2015/19/B/ST7/02984]; Innovative Economy Operational Programme (POIG) [02.01.00-00-166/08, POIG. 02.03.01-00040/13]

Available from: 2017-05-09 Created: 2017-05-09 Last updated: 2017-08-17Bibliographically approved

Open Access in DiVA

fulltext(2139 kB)0 downloads
File information
File name FULLTEXT01.pdfFile size 2139 kBChecksum SHA-512
e312ca1d0eee2006b0ba9fd70edf9239dbc221b81b1fa8d88ef35708c2cddd70b87e9cafdfc73509b95b8819c79adba71a0a0ae2783aaef0d94694b5e094e2ae
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMed

Authority records BETA

Wiechec, Emilia

Search in DiVA

By author/editor
Wiechec, Emilia
By organisation
Division of Speech language pathology, Audiology and OtorhinolaryngologyFaculty of Medicine and Health Sciences
In the same journal
Medicinal chemistry
Health Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 91 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf