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Neutravidin biosensor for direct capture of dual-functional biotin-molecular beacon-AuNP probe for sensitive voltammetric detection of microRNA
Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering. University of Sousse, Higher Institute of Applied Sciences and Technology of Sousse, GREENS-ISSAT, Cité Ettafala, 4003 Ibn Khaldoun Sousse, Tunisia.
Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering. University of Sousse, Higher Institute of Applied Sciences and Technology of Sousse, GREENS-ISSAT, Cité Ettafala, 4003 Ibn Khaldoun Sousse, Tunisia.
Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering.ORCID iD: 0000-0002-1815-9699
University of Sousse, Higher Institute of Applied Sciences and Technology of Sousse, GREENS-ISSAT, Cité Ettafala, 4003 Ibn Khaldoun Sousse, Tunisia.
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2017 (English)In: Sensors and actuators. B, Chemical, ISSN 0925-4005, E-ISSN 1873-3077, Vol. 248, 8 p.77-84 p.Article in journal (Refereed) Published
Abstract [en]

We have demonstrated a new approach using a neutravidin-based biosensor combined with a dual-function gold nanoparticle (AuNP) biolabel, for simple and sensitive detection of microRNA-21 (miRNA-21). The selectivity of the biosensor is provided by the intrinsic properties of the dual-functional biotin-MB-AuNP label. The assay procedure is relatively simple, exploiting a one-pot assay concept where the affinity capture of the miRNA-21/dual-functional biotin-MB-AuNP complex, via the strong biotin-neutravidin supramolecular interaction, and simultaneous detection of the captured AuNPs label with stripping voltammetry, is performed in a single step. This electrochemical miRNA biosensor could detect miRNA-21 with limit of detection of 0.1×10less thansuperscriptgreater than−12less than/superscriptgreater than and a dynamic range from 0.5×10less thansuperscriptgreater than−12less than/superscriptgreater than to 1.0×10less thansuperscriptgreater than−9less than/superscriptgreater thanM. The performance of the miRNA-21biosensor was further improved after silver deposition onto the AuNPs, delivering an enhanced detection limit of 4.0×10less thansuperscriptgreater than−15less than/superscriptgreater thanM of miRNA-21, and an extremely wide analytic dynamic range from 10×10less thansuperscriptgreater than−15less than/superscriptgreater than to 1×10less thansuperscriptgreater than−9less than/superscriptgreater thanM (5 orders of magnitude). This exceptionally broad dynamic range demonstrates the advantage of the one-pot assay approach with direct capture of the dual functional biotin-MB-AuNP via the strong biotin-neutravidin supramolecular interaction. Furthermore, we demonstrated the detection of miRNA-21 in spiked serum at clinically relevant concentrations. The miRNA biosensor displayed excellent analytical performance for the detection of miRNA and could provide a powerful and convenient tool for biomedical research and applications in cancer diagnostics.

Place, publisher, year, edition, pages
Elsevier, 2017. Vol. 248, 8 p.77-84 p.
National Category
Analytical Chemistry
Identifiers
URN: urn:nbn:se:liu:diva-137576DOI: 10.1016/j.snb.2017.03.160OAI: oai:DiVA.org:liu-137576DiVA: diva2:1097104
Available from: 2017-05-22 Created: 2017-05-22 Last updated: 2017-05-22

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The full text will be freely available from 2019-03-31 10:53
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CiteExportLink to record
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