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Specific Imaging of Intracellular Lipid Droplets Using a Benzothiadiazole Derivative with Solvatochromic Properties
Linköping University, Department of Physics, Chemistry and Biology, Chemistry. Linköping University, Faculty of Science & Engineering.
University of Gothenburg, Sweden.
University of Gothenburg, Sweden.
Linköping University, Department of Physics, Chemistry and Biology, Chemistry. Linköping University, Faculty of Science & Engineering.ORCID iD: 0000-0002-5582-140X
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2017 (English)In: Bioconjugate chemistry, ISSN 1043-1802, E-ISSN 1520-4812, Vol. 28, no 5, 1363-1370 p.Article in journal (Refereed) Published
Abstract [en]

Altered lipid metabolism and extensive lipid storage in cells have been associated with various medical disorders, including cancer. The development of fluorescent probes that specifically accumulate in lipid deposits is therefore of great interest in order to study pathological processes that are linked to dysregulated lipogenesis. In the present study, we present a small fluorescent benzothiadiazole dye that specifically stains lipid droplets in living and fixated cells. The photophysical characterization of the probe revealed strong solvatochromic behavior, large Stokes shifts, and high fluorescent quantum yields in hydrophobic solvents. In addition, the fluorophore exhibits a nontoxic profile and a high signal-to-noise ratio in cells (i.e., lipid droplets vs cytosol), which make it an excellent candidate for studying lipid biology using confocal fluorescent microscopy.

Place, publisher, year, edition, pages
AMER CHEMICAL SOC , 2017. Vol. 28, no 5, 1363-1370 p.
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:liu:diva-138482DOI: 10.1021/acs.bioconjchem.7b00048ISI: 000401782000005PubMedID: 28402621OAI: oai:DiVA.org:liu-138482DiVA: diva2:1111672
Note

Funding Agencies|Swedish Research Council [350-2012-239]; Swedish Foundation of Strategic Research [ICA14-0018]; Wenner-Gren Foundation

Available from: 2017-06-19 Created: 2017-06-19 Last updated: 2017-07-10

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The full text will be freely available from 2018-04-12 14:34
Available from 2018-04-12 14:34

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Appelqvist, HannaNilsson, PeterDyrager, Christine
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