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Early life stress and its association with epigenetics and immune system response
Linköping University, Department of Physics, Chemistry and Biology, Biology.
2017 (English)Independent thesis Advanced level (degree of Master (Two Years)), 40 credits / 60 HE creditsStudent thesis
Abstract [en]

Stress can induce prolonged deleterious effects on many characteristics in chickens (Gallus gallus). Particular interest has been paid to early life stress. Social isolation as an early life stressor results in increased plasma corticosterone levels. Moreover, it induces behavioural and physiological changes as well as gene expression modifications in the hypothalamus. In the first part of my study, I aim to inquire into social isolation impacts on the short and long-term. Short and long-term effects were assessed by immune system, behaviour and weight. 82 male chickens were assigned to three groups (stress, control and enrichment). The stress group was exposed to social isolation, the enrichment group was provided with enrichment substrates while the control group was left untreated. According to my knowledge, this is the first study that investigates the effects of social isolation on the interuleikn-6 levels as an indicator of immune system response. My findings suggest that social isolation induces short and long-term effects on immune response as well as on body weight. In the second part of my study, I aim to develop a method investigating effects of early stress on DNA methylation in blood and sperm. For this purpose, two methods GBS (Genotyping by sequencing) and MeDIP (Methylated DNA immuneprecipitation) were f using pooled DNA from all individuals for the first time. Moreover, I developed a protocol for extracting sperm DNA from frozen testis. Combining both methods has many advantages, such as cost effectiveness and the ability to evaluate epigenetic signatures in large number of individuals

Place, publisher, year, edition, pages
2017. , p. 51
Keywords [en]
DNA methylation, Epigenetic, GBS, Interleukin-6, MeDIP, Stress, social isolation, sperms
National Category
Behavioral Sciences Biology
Identifiers
URN: urn:nbn:se:liu:diva-138947ISRN: LITH-IFM-A-EX--17/3346--SEOAI: oai:DiVA.org:liu-138947DiVA, id: diva2:1115433
Subject / course
Biology
Presentation
2017-05-24, Nobel, Linköping, 15:30 (English)
Examiners
Available from: 2017-06-27 Created: 2017-06-26 Last updated: 2017-06-27Bibliographically approved

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CiteExportLink to record
Permanent link

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Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf