liu.seSearch for publications in DiVA
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Amperometric L-arginine biosensor based on a novel recombinant arginine deiminase
National Academic Science Ukraine, Ukraine.
NAS Ukraine, Ukraine.
Lviv State University of Phys Culture, Ukraine.
NAS Ukraine, Ukraine.
Show others and affiliations
2017 (English)In: Microchimica Acta, ISSN 0026-3672, E-ISSN 1436-5073, Vol. 184, no 8, 2679-2686 p.Article in journal (Refereed) Published
Abstract [en]

The authors describe an amperometric biosensor for the amino acid L-arginine (L-Arg). It is based on the use of a Nafion/Polyaniline (PANi) composite on a platinum screen-printed electrode (Pt-SPE) using a novel recombinant arginine deiminase isolated from Mycoplasma hominis. The protein was over-expressed, purified and employed as a biorecognition element of the sensor. Enzymatic hydrolysis of L-Arg leads to the formation of ammonium ions which diffuse into the Nafion/PANi layer and induce the electroreduction of PANi at a potential of -0.35 V (vs Ag/AgCl). L-Arg sensitivity is 684 +/- 32 A.M-1.m(-2), and the apparent Michaelis-Menten constant K-M(app)) is 0.31 +/- 0.05 mM. The calibration plot is linear over the range 3-200 mu M L-Arg, the limit of detection is 1 mu M, and the response time (for 90% of the total signal change to occur) is 15 s. The sensor is selective and exhibits good storage stability (amp;gt; 1 month without loss in signal). The biosensor was applied to the analysis of L-Arg in pharmaceutical samples and of ammonium and L-Arg in spiked human plasma obtained from blood of healthy volunteers and those with a hepatic disorder. Data generated were found to be in good agreement with a reference fluorometric enzymatic assay.

Place, publisher, year, edition, pages
SPRINGER WIEN , 2017. Vol. 184, no 8, 2679-2686 p.
Keyword [en]
L-arginine; Ammonium ions; Amperometry; Nafion; Polyaniline; Recombinant enzyme; Plasma samples; Pharmaceuticals
National Category
Analytical Chemistry
Identifiers
URN: urn:nbn:se:liu:diva-139518DOI: 10.1007/s00604-017-2290-4ISI: 000406066500021OAI: oai:DiVA.org:liu-139518DiVA: diva2:1130196
Note

Funding Agencies|European Community [PIRSES-GA-2012-318053]; NATO Science for Peace (SFP) [CBP.NUKR.SFPP 984173]

Available from: 2017-08-08 Created: 2017-08-08 Last updated: 2017-08-30

Open Access in DiVA

The full text will be freely available from 2018-05-02 12:48
Available from 2018-05-02 12:48

Other links

Publisher's full text

Search in DiVA

By author/editor
Turner, Anthony
By organisation
Biosensors and BioelectronicsFaculty of Science & Engineering
In the same journal
Microchimica Acta
Analytical Chemistry

Search outside of DiVA

GoogleGoogle Scholar

Altmetric score

Total: 55 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf