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Establishing and validating the fluorescent amyloid ligand h-FTAA (heptamer formyl thiophene acetic acid) to identify transthyretin amyloid deposits in carpal tunnel syndrome
Christian Albrechts University of Kiel, Germany.
Linköping University, Department of Physics, Chemistry and Biology, Chemistry. Linköping University, Faculty of Science & Engineering.ORCID iD: 0000-0002-5582-140X
Linköping University, Department of Physics, Chemistry and Biology, Chemistry. Linköping University, Faculty of Science & Engineering.
Institute Pathol and Dermatopathol, Germany.
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2017 (English)In: Amyloid: Journal of Protein Folding Disorders, ISSN 1350-6129, E-ISSN 1744-2818, Vol. 24, no 2, 78-86 p.Article in journal (Refereed) Published
Abstract [en]

Transthyretin-derived (ATTR) amyloidosis is a frequent finding in carpal tunnel syndrome. We tested the following hypotheses: the novel fluorescent amyloid ligand heptameric formic thiophene acetic acid (h-FTAA) has a superior sensitivity for the detection of amyloid compared with Congo red-staining; Amyloid load correlates with patient gender and/or patient age. We retrieved 208 resection specimens obtained from 184 patients with ATTR amyloid in the carpal tunnel. Serial sections were stained with Congo red, h-FTAA and an antibody directed against transthyretin (TTR). Stained sections were digitalized and forwarded to computational analyses. The amount of amyloid was correlated with patient demographics. Amyloid stained intensely with h-FTAA and an anti-TTR-antibody. Congo red-staining combined with fluorescence microscopy was significantly less sensitive than h-FTAA-fluorescence and TTR-immunostaining: the highest percentage area was found in TTR-immunostained sections, followed by h-FTAA and Congo red. The Pearson correlation coefficient was .8 (Congo red vs. h-FTAA) and .9 (TTR vs. h-FTAA). Amyloid load correlated with patient gender, anatomical site and patient age. h-FTAA is a highly sensitive method to detect even small amounts of ATTR amyloid in the carpal tunnel. The staining protocol is easy and h-FTAA may be a much more sensitive procedure to detect amyloid at an earlier stage.

Place, publisher, year, edition, pages
TAYLOR & FRANCIS LTD , 2017. Vol. 24, no 2, 78-86 p.
Keyword [en]
Amyloid; Congo red; ATTR; transthyretin; h-FTAA; carpal tunnel
National Category
Gastroenterology and Hepatology
Identifiers
URN: urn:nbn:se:liu:diva-139829DOI: 10.1080/13506129.2017.1316711ISI: 000406234900002PubMedID: 28434259OAI: oai:DiVA.org:liu-139829DiVA: diva2:1133894
Note

Funding Agencies|German Research Foundation [Ro 1173/11]; Federal Ministry of Education and Research (GERAMY)

Available from: 2017-08-17 Created: 2017-08-17 Last updated: 2017-08-17

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