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Visible, Hyperspectral Imaging Evaluating the Cutaneous Response to Ultraviolet Radiation
Linköping University, Department of Biomedical Engineering, Biomedical Instrumentation. Linköping University, The Institute of Technology.
Linköping University, Department of Biomedical Engineering, Biomedical Instrumentation. Linköping University, The Institute of Technology.
Linköping University, Department of Biomedicine and Surgery, Division of dermatology and venereology. Linköping University, Faculty of Health Sciences. Östergötlands Läns Landsting, Centre for Medicine, Department of Dermatology and Venerology UHL.
Linköping University, Department of Biomedical Engineering, Biomedical Instrumentation. Linköping University, The Institute of Technology.
2007 (English)In: Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V / [ed] Daniel L. Farkas; Robert C. Leif; Dan V. Nicolau, SPIE - International Society for Optical Engineering, 2007, 644103-1-644103-12 p.Conference paper, Published paper (Other academic)
Abstract [en]

In vivo diagnostics of skin diseases as well as understanding of the skin biology constitute a field demanding characterization of physiological and anatomical parameters. Biomedical optics has been successfully used, to qualitatively and quantitatively estimate the microcirculatory conditions of superficial skin. Capillaroscopy, laser Doppler techniques and spectroscopy, all elucidate different aspects of microcirculation, e.g. capillary anatomy and distribution, tissue perfusion and hemoglobin oxygenation. We demonstrate the use of a diffuse reflectance hyperspectral imaging system for spatial and temporal characterization of tissue oxygenation, important to skin viability. The system comprises: light source, liquid crystal tunable filter, camera objective, CCD camera, and the decomposition of the spectral signature into relative amounts of oxy- and deoxygenized hemoglobin as well as melanin in every pixel resulting in tissue chromophore images. To validate the system, we used a phototesting model, creating a graded inflammatory response of a known geometry, in order to evaluate the ability to register spatially resolved reflectance spectra. The obtained results demonstrate the possibility to describe the UV inflammatory response by calculating the change in tissue oxygen level, intimately connected to a tissue's metabolism. Preliminary results on the estimation of melanin content are also presented.

Place, publisher, year, edition, pages
SPIE - International Society for Optical Engineering, 2007. 644103-1-644103-12 p.
Series
Proceedings of SPIE (Progress in biomedical optics and imaging), ISSN 1605-7422 ; 6441
Keyword [en]
Hyperspectral imaging, Ultraviolet provocation, Erythema, Hemoglobin, CCD camera, Tunable filters
National Category
Medical Laboratory and Measurements Technologies
Identifiers
URN: urn:nbn:se:liu:diva-15190DOI: 10.1117/12.698165ISI: 000245855200002ISBN: 9780819465542 (print)OAI: oai:DiVA.org:liu-15190DiVA: diva2:113602
Conference
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V, 20 January 2007, San Jose, CA, USA
Available from: 2008-10-22 Created: 2008-10-22 Last updated: 2014-01-30Bibliographically approved
In thesis
1. In Vivo Diffuse Reflectance Spectroscopy of Human Tissue: From Point Measurements to Imaging
Open this publication in new window or tab >>In Vivo Diffuse Reflectance Spectroscopy of Human Tissue: From Point Measurements to Imaging
2008 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

This thesis presents the non-invasive use of diffuse reflectance spectroscopy (DRS) to provide information about the biochemical composition of living tissue. During DRS measurements, the incident, visible light is partially absorbed by chromophores but also scattered in the tissue before being remitted.

Human skin and heart, the main tissue objects in this thesis, are dependent on a sufficient inflow of oxygenized blood, and outflow of metabolic byproducts. This process could be monitored by DRS using the spectral fingerprints of the most important tissue chromophores, oxyhemoglobin and deoxyhemoglobin.

The Beer-Lambert law was used to produce models for the DRS and has thus been a foundation for the analyses throughout this work. Decomposition into the different chromophores was performed using least square fitting and tabulated data for chromophore absorptivity.

These techniques were used to study skin tissue erythema induced by a provocation of an applied heat load on EMLA-treated skin. The absorbance differences, attributed to changes in the hemoglobin concentrations, were examined and found to be related to, foremost, an increase in oxyhemoglobin.

To estimate UV-induced border zones between provoked and nonprovoked tissue a modified Beer-Lambert model, approximating the scattering effects, was used. An increase of chromophore content of more than two standard deviations above mean indicated responsive tissue. The analysis revealed an edge with a rather diffuse border, contradictory to the irradiation pattern.

Measuring in the operating theater, on the heart, it was necessary to calculate absolute chromophore values in order to assess the state of the myocardium. Therefore, a light transport model accounting for the optical properties, and a calibrated probe, was adopted and used. The absolute values and fractions of the chromophores could then be compared between sites and individuals, despite any difference of the optical properties in the tissue.

A hyperspectral imaging system was developed to visualize the spatial distribution of chromophores related to UV-provocations. A modified Beer-Lambert approximation was used including the chromophores and a baseline as an approximate scattering effect. The increase in chromophore content was estimated and evaluated over 336 hours.

In conclusion, advancing from a restricted Beer-Lambert model, into a model estimating the tissue optical properties, chromophore estimation algorithms have been refined progressively. This has allowed advancement from relative chromophore analysis to absolute values, enabling precise comparisons and good prediction of physiological conditions.

Place, publisher, year, edition, pages
Linköping: Linköping University Electronic Press, 2008. 88 p.
Series
Linköping Studies in Science and Technology. Dissertations, ISSN 0345-7524 ; 1210
National Category
Medical Laboratory and Measurements Technologies
Identifiers
urn:nbn:se:liu:diva-15191 (URN)978-91-7393-809-9 (ISBN)
Public defence
2008-10-31, Elsa Brändströmsalen, Södra entrén, Campus US, Universitetssjukhuset, Linköpings universitet, Linköping, 09:00 (English)
Opponent
Supervisors
Available from: 2008-10-22 Created: 2008-10-22 Last updated: 2009-04-30Bibliographically approved

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Ilias, Michail A.Häggblad, ErikAnderson, ChrisSalerud, Göran

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Ilias, Michail A.Häggblad, ErikAnderson, ChrisSalerud, Göran
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Biomedical InstrumentationThe Institute of TechnologyDivision of dermatology and venereologyFaculty of Health SciencesDepartment of Dermatology and Venerology UHL
Medical Laboratory and Measurements Technologies

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