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Substantial discrepancies in 17β-estradiol concentrations obtained with three different commercial direct radioimmunoassay kits in rat sera
Linköping University, Department of Clinical and Experimental Medicine. Linköping University, Faculty of Health Sciences.
Linköping University, Department of Clinical and Experimental Medicine, Clinical Chemistry. Linköping University, Faculty of Health Sciences. Östergötlands Läns Landsting, Reconstruction Centre, Department of Neurosurgery UHL.
Linköping University, Department of Clinical and Experimental Medicine, Clinical Chemistry. Linköping University, Faculty of Health Sciences. Östergötlands Läns Landsting, Centre for Laboratory Medicine, Department of Clinical Chemistry.
2008 (English)In: Scandinavian Journal of Clinical and Laboratory Investigation, ISSN 0036-5513, E-ISSN 1502-7686, Vol. 68, no 8, 806-813 p.Article in journal (Refereed) Published
Abstract [en]

The extensive use of estrogen for contraception and amelioration of post-menopausal symptoms has made it the subject of substantial recent research efforts. Ovariectomized (ovx) rats treated with exogenous ovarial hormones constitute important tools in the investigation of the effects and mechanisms of estrogen actions. The crucial need to control and to monitor plasma levels of 17β-estradiol calls for accurate, precise and robust assay methods. The performance of direct radioimmunoassays (RIAs) for measurement of 17β-estradiol has previously been reported for human samples, but – to our knowledge – not for rat samples. In the current study, 552 serum samples from ovx, native and hormone treated rats were used to compare the performance of three commercially manufactured direct RIAs from the companies DPC (Siemens Healthcare Diagnostics Inc., formerly Diagnostic Products Corporation), DSL (Diagnostic Systems Labs) and MPB (MP Biomedicals, formerly ICN Biomedicals). Substantial differences in results between the three assay methods were found when measuring serum 17β-estradiol concentrations. The following formulas, describing the relation between the different methods’ results, were obtained using weighted Deming’s orthogonal regression (all regression formulae in the abstract are based on pg/mL): DSL= 0.43*DPC+12.3, MPB= 2.1*DPC+84.7 and DSL= 4.8*MPB+22.2. Furthermore, a preceding diethyl ether extraction step of the serum appears to impair the RIAs’ performances in the present samples: DPCex= 0.39*DPCunex+0.76, DSLex= 0.32*DSLunex-1.7 and MPBex= 0.22*MPBunex+1.4.

Place, publisher, year, edition, pages
2008. Vol. 68, no 8, 806-813 p.
Keyword [en]
Estrogens, Validation Studies, Pharmacokinetics, Radioimmunoassay, Rats
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:liu:diva-15392DOI: 10.1080/00365510802254638OAI: oai:DiVA.org:liu-15392DiVA: diva2:114094
Note
Original publication: Jakob O. Ström, Annette Theodorsson and Elvar Theodorsson, Substantial discrepancies in 17β-estradiol concentrations obtained with three different commercial direct radioimmunoassay kits in rat sera, 2008, Scandinavian Journal of Clinical and Laboratory Investigation.http://dx.doi.org/10.1080/00365510802254638. Copyright © Taylor & Francis Group, an informa businessAvailable from: 2008-11-05 Created: 2008-11-05 Last updated: 2017-12-14Bibliographically approved

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Ström, Jakob O.Theodorsson, AnnetteTheodorsson, Elvar

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