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RNAi-mediated gene silencing in Rhynchophorus ferrugineus (Oliver) (Coleoptera: Curculionidae)
University of Mediterranea Reggio Calabria, Italy.
University of Mediterranea Reggio Calabria, Italy.
Newcastle University, England.
Linköping University, Department of Physics, Chemistry and Biology, Biology. Linköping University, Faculty of Science & Engineering. University of Mediterranea Reggio Calabria, Italy.ORCID iD: 0000-0002-0811-1229
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2017 (English)In: Open Life Sciences, ISSN 2391-5412, Vol. 12, no 1, p. 214-222Article in journal (Refereed) Published
Abstract [en]

RNA interference (RNAi) is a powerful strategy for gene function analysis, and it is also widely studied in view of a promising use in pest control. The red palm weevil, Rhynchophorus ferrugineus (Oliver) (Coleoptera: Curculionidae), is one of the most devastating pests of palm in the world. Conventional pest management practices are not adequate to control this insect, thus the development of efficient approaches with minimal environmental impact are needed. In this work, the potential of RNAi in R. ferrugineus has been investigated through the silencing of three different genes (alpha-amylase, V-ATPase, Ecdysone receptor). For each gene we tested two different doses (1,500 and 5,500 ng) and two delivery techniques (injection and ingestion), evaluating both gene knockdown and mortality on insects. Results show that RNAi mediated gene silencing in R. ferrugineus varies from gene to gene, and that the response is dose-dependent, with stronger effects when dsRNA was administered by injection. In parallel, the same study was carried out with the model organism Tribolium castaneum Herbst (Coleoptera: Tenebrionidae), with results showing a different pattern of response, although the two insects belong to the same order.

Place, publisher, year, edition, pages
De Gruyter Open, 2017. Vol. 12, no 1, p. 214-222
Keywords [en]
alpha-amylase; Ecdysone receptor; dsRNA; injection; oral delivery; RNA interference; V-ATPase
National Category
Genetics
Identifiers
URN: urn:nbn:se:liu:diva-140980DOI: 10.1515/biol-2017-0025ISI: 000408793400001Scopus ID: 2-s2.0-85028851498OAI: oai:DiVA.org:liu-140980DiVA, id: diva2:1142300
Available from: 2017-09-19 Created: 2017-09-19 Last updated: 2018-04-26Bibliographically approved

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