liu.seSearch for publications in DiVA
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Coronin 1C negatively regulates cell-matrix adhesion and motility of intestinal epithelial cells
Epithelial Pathobiology Research Unit, Department of Pathology and Laboratory Medicine, Emory University, Atlanta, GA, USA.
Linköping University, Department of Clinical and Experimental Medicine, Division of Microbiology and Molecular Medicine. Linköping University, Faculty of Medicine and Health Sciences. Epithelial Pathobiology Research Unit, Department of Pathology and Laboratory Medicine, Emory University, Atlanta, GA, USA.
Department of Medicine, University of Rochester, Rochester, NY, USA.
Epithelial Pathobiology Research Unit, Department of Pathology and Laboratory Medicine, Emory University, Atlanta, GA, USA.
Show others and affiliations
2010 (English)In: Biochemical and Biophysical Research Communications - BBRC, ISSN 0006-291X, E-ISSN 1090-2104, Vol. 391, no 1, 394-400 p.Article in journal (Refereed) Published
Abstract [en]

Coronins, WD-repeat actin-binding proteins, are known to regulate cell motility by coordinating actin filament turnover in lamellipodia of migrating cell. Here we report a novel mechanism of Coronin 1C-mediated cell motility that involves regulation of cell-matrix adhesion. RNAi silencing of Coronin 1C in intestinal epithelial cells enhanced cell migration and modulated lamellipodia dynamics by increasing the persistence of lamellipodial protrusion. Coronin 1C-depleted cells showed increased cell-matrix adhesions and enhanced cell spreading compared to control cells, while over-expression of Coronin 1C antagonized cell adhesion and spreading. Enhanced cell-matrix adhesion of coronin-deficient cells correlated with hyperphosphorylation of focal adhesion kinase (FAK) and paxillin, and an increase in number of focal adhesions and their redistribution at the cell periphery. siRNA depletion of FAK in coronin-deficient cells rescued the effects of Coronin 1C depletion on motility, cell-matrix adhesion, and spreading. Thus, our findings provide the first evidence that Coronin 1C negatively regulates epithelial cell migration via FAK-mediated inhibition of cell-matrix adhesion.

Place, publisher, year, edition, pages
Elsevier, 2010. Vol. 391, no 1, 394-400 p.
Keyword [en]
Coronin; FAK; Motility; Adhesion
National Category
Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:liu:diva-141668DOI: 10.1016/j.bbrc.2009.11.069PubMedID: 19913511ISBN: 1090-2104 (Electronic) 0006-291X (Linking) OAI: oai:DiVA.org:liu-141668DiVA: diva2:1149698
Note

Samarin, Stanislav N Koch, Stefan Ivanov, Andrei I Parkos, Charles A Nusrat, Asma eng R01 DK059888-09/DK/NIDDK NIH HHS/ R01 DK061379-08/DK/NIDDK NIH HHS/ R29 DK055679/DK/NIDDK NIH HHS/ DK 55679/DK/NIDDK NIH HHS/ DK 064399/DK/NIDDK NIH HHS/ DK 61379/DK/NIDDK NIH HHS/ R01 DK072564/DK/NIDDK NIH HHS/ DK 72564/DK/NIDDK NIH HHS/ R24 DK064399/DK/NIDDK NIH HHS/ R01 DK055679-11A1/DK/NIDDK NIH HHS/ R24 DK064399-019003/DK/NIDDK NIH HHS/ R01 DK055679/DK/NIDDK NIH HHS/ DK 59888/DK/NIDDK NIH HHS/ R01 DK061379/DK/NIDDK NIH HHS/ R01 DK059888/DK/NIDDK NIH HHS/ R01 DK072564-15/DK/NIDDK NIH HHS/ Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't 2009/11/17 06:00 Biochem Biophys Res Commun. 2010 Jan 1;391(1):394-400. doi: 10.1016/j.bbrc.2009.11.069. Epub 2009 Nov 12.

Available from: 2017-10-16 Created: 2017-10-16 Last updated: 2017-10-24Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Authority records BETA

Koch, Stefan

Search in DiVA

By author/editor
Koch, Stefan
By organisation
Division of Microbiology and Molecular MedicineFaculty of Medicine and Health Sciences
In the same journal
Biochemical and Biophysical Research Communications - BBRC
Cell and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar

doi
pubmed
isbn
urn-nbn

Altmetric score

doi
pubmed
isbn
urn-nbn
Total: 52 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf